Effects of cadmium of the human placenta in vitro

Thesis/Dissertation · Tue Jan 01 00:00:00 EST 1985

OSTI ID:6705189

Wier, P J

Human placental lobules (25 g) were maintained in vitro for 12 hours by dual perfusion of the fetal vessels and intervillous space (maternal circulation). A synthetic tissue culture medium was used for the perfusates. Maternal perfusate was gassed with 95% O/sub 2//5% CO/sub 2/; fetal perfusate was gassed with 95% N/sub 2//5% CO/sub 2/. Both perfusates were recirculated (maternal 15-25 ml/min, fetal 3 ml/min) and exchanged for fresh perfusates every 4 hours. The integrity of the fetal vasculature was demonstrated by the stabilities of fetal arterial pressure (30-36 mm Hg) and circulatory volume (volume loss <2 ml/h). The metabolic viability of the placenta was documented by measurements of oxygen consumption (100-137 umole/min.kg), glucose consumption (133-163 umole/min.kg), and lactate production (163-229 umole/min.kg). Exposure of placenta to 10 nmole Cd/ml maternal perfusate led to accumulation of cadmium (45 nmole Cd/g), yet there was limited movement of cadmium from maternal to fetal circulations. This exposure did not result in significant changes in fetal capillary permeability of resistance, oxygen or glucose consumptions, lactate production, nCg release, transport of AlB by placental slices, or ultrastructure. Transfer of zinc from maternal to fetal circulations did not appear to be reduced. Exposure of perfused placenta to 100 nmole Cd/ml maternal perfusate resulted in a cadmium burden of 150 nmole Cd/g. This exposure induced syncytiotrophoblast necrosis, increased fetal capillary permeability, and reduced hCG release.

• Research Organization: Rochester Univ., NY (USA)
• OSTI ID: 6705189
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CADMIUM
BIOLOGICAL EFFECTS
ZINC
UPTAKE
CYCLOHEXIMIDE
HCG
IN VITRO
PERFUSED ORGANS
PLACENTA
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BODY
DRUGS
ELEMENTS
FETAL MEMBRANES
FUNGICIDES
GONADOTROPINS
HORMONES
MEMBRANES
METALS
ORGANS
PEPTIDE HORMONES
PESTICIDES
PITUITARY HORMONES
550000* - Biomedical Sciences
Basic Studies
560300 - Chemicals Metabolism &amp; Toxicology