Prevention of GVHD by modulation of rat bone marrow with UV-B irradiation. II. Kinetics of migration of UV-B-irradiated bone marrow cells in naive and lethally irradiated rats

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Cellular Immunology; (USA)

DOI:https://doi.org/10.1016/0008-8749(90)90026-N· OSTI ID:6704661

Oluwole, S F; Engelstad, K; Hardy, M A ^[1]

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1. Columbia Univ. College of Physicians and Surgeons, New York, NY (USA)

UV-B irradiation (700 J/m2) of bone marrow (BM) cells prior to transplantation into lethally gamma-irradiated (1050 rad) allogeneic rats prevents the development of GVHD and results in a stable mixed lymphohematopoietic chimerism. To better understand the underlying mechanisms of the development of stable radiation chimeras in this model, this study was designed to examine whether the dose (700 J/m2) of UV-B irradiation used for the modulation of the BM inoculum would affect the homing pattern of radiolabeled BM cells compared to that of thoracic duct lymphocytes (TDL) in the naive and lethally irradiated recipients. The results showed that intravenously administered, 111Indium-oxine-labeled, unmodified TDL home specifically to the spleen, lymph nodes, and BM compartments with a subsequent recirculation of a large number of cells from the spleen to the lymph nodes. In contrast, radiolabeled, unmodified BM cells migrate specifically to the spleen, liver, and BM with the lymph nodes, thymus, and nonlymphoid organs containing very little amounts of radioactivity. The stable concentrations of radioactivity in the lymphoid and nonlymphoid compartments between 3 and 72 hr after injection suggest that BM cells, unlike TDL, do not recirculate. The migration pattern of BM cells in the naive recipient was not significantly different from that seen in lethally irradiated animals except for the higher concentration of radioactivity in the spleen and BM of irradiated animals compared to that seen in naive recipients. The similarity of tissue localization of BM cells in naive or in irradiated syngeneic recipients to that of allogeneic recipients suggests that the homing of BM cells is not MHC restricted.

• OSTI ID: 6704661
• Journal Information: Cellular Immunology; (USA), Vol. 128:1; ISSN 0008-8749
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BONE MARROW CELLS
TRANSPLANTS
BIOLOGICAL RADIATION EFFECTS
GAMMA RADIATION
GRAFT-HOST REACTION
INDIUM 111
LYMPH NODES
LYMPHOCYTES
MIGRATION
RADIATION CHIMERAS
RATS
SPLEEN
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHIMERAS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
MOSAICISM
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
560152 - Radiation Effects on Animals- Animals
550901 - Pathology- Tracer Techniques