Quantitative reverse sample genome probing of microbial communities and its application to oil field production waters

Journal Article · Wed Dec 01 00:00:00 EST 1993 · Applied and Environmental Microbiology; (United States)

OSTI ID:6703677

Voordouw, G; Shen, Y; Harrington, C S; Teland, A J ^[1]; Jack, T R ^[2]; Westlake, W S ^[3]

---

1. Univ. of Calgary, Alberta (Canada)
2. Novacor Research and Technology Corporation, Calgary, Alberta (Canada)
3. Univ. of Alberta (Canada)

This paper presents a protocol for quantitative analysis of microbial communities by reverse sample genome probing is presented in which (i) whole community DNA is isolated and labeled in the presence of a known amount of an added internal standard and (ii) the resulting spiked reverse genome probe is hybridized with a master filter on which denatured genomic DNAs from bacterial standards isolated from the target environment were spotted in large amounts (up to 1,500 ng) in order to improve detection sensitivity. This protocol allowed reproducible fingerprinting of the microbial community in oil field production waters at 19 sites from which water and biofilm samples were collected. It appeared that selected sulfate-reducing bacteria were significantly enhanced in biofilms covering the metal surfaces in contact with the production waters.

• OSTI ID: 6703677
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 59:12; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
02 PETROLEUM
DNA
EVALUATION
MICROORGANISMS
GENES
OIL FIELDS
PATTERN RECOGNITION
PROBES
GEOLOGIC DEPOSITS
MINERAL RESOURCES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PETROLEUM DEPOSITS
RESOURCES
560300* - Chemicals Metabolism & Toxicology
020000 - Petroleum