Fluorescence photobleaching measurements of plant membrane viscosity: Effects of environmental stress: Final report

Technical Report · Sat Nov 01 00:00:00 EST 1986

OSTI ID:6703280

Breidenbach, R W

The primary purpose of this project was to measure the temperature dependence of the fluidity of plant cell membranes by fluorescence photobleaching recovery, and see if there is a change in fluidity at the temperature of chilling injury. For practical reasons, we have concentrated on measurements of the lateral diffusion constant of the lipid analog dil in tomato vacuolar membrane. We have found an apparent change in the diffusion constant of dil near the temperature of chilling injury (approx.12/sup 0/C), but the scatter in the diffusion constants makes it difficult to prove that there is a change. 11 refs.

• Research Organization: California Univ., Davis (USA). Plant Growth Lab.
• DOE Contract Number: AT03-80ER10700
• OSTI ID: 6703280
• Report Number(s): DOE/ER/10700-T3; ON: DE87007534
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CELL MEMBRANES
VISCOSITY
OLIGOSACCHARIDES
BIOLOGICAL FUNCTIONS
BIOPHYSICS
CELL CYCLE
FREEZING
PLANT CELLS
TEMPERATURE DEPENDENCE
CARBOHYDRATES
CELL CONSTITUENTS
FUNCTIONS
MEMBRANES
ORGANIC COMPOUNDS
SACCHARIDES
550300* - Cytology
560400 - Other Environmental Pollutant Effects