Inhibition of X-ray-induced potentially lethal damage (PLD) repair by cordycepin (3'-deoxyadenosine) and enhancement of its action by 2'-deoxycoformycin in Chinese Hamster hai cells in the stationary phase in Vitro
The effects of growth phase and chemicals on PLD repair were studied in X-irradiated Chinese hamster hai cells. The change in capacity of cells in different growth phases to repair PLD was investigated. Starting from cells in the log phase, the magnitude of PLD repair during 10 hr of postirradiation incubation in Hanks' balanced salt solution increased for 2.5 to 18 as the cultures approached the stationary phase, which occurred on the 7th or 8th day. The effects of chemicals dissolved in Hanks' BSS on PLD repair were studied using 10th- or 12th-day cultures. Among the chemicals tested, caffeine and cordycepin were effective in inhibiting PLD repair. When 2'-deoxycoformycin, an inhibitor of adenosine deaminase, was combined with cordycepin, the effect of cordycepin was enhanced. Due to this prevention of the deamination of cordycepin by 2'-deoxycoformycin, the inhibition of PLD repair was prolonged, indicating a possible clinical application of cordycepin as a radiosensitizer.
- Research Organization:
- Kyoto Univ. (Japan)
- OSTI ID:
- 6701055
- Journal Information:
- Radiat. Res.; (United States), Vol. 84:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL REPAIR
CORRELATIONS
CELL CYCLE
CELL KILLING
RADIOINDUCTION
ADENOSINE
CAFFEINE
ENZYME INHIBITORS
IN VITRO
INHIBITION
X RADIATION
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RIBOSIDES
XANTHINES
560121* - Radiation Effects on Cells- External Source- (-1987)