Estrogenic regulation of Leydig cell development in the rat

Miscellaneous · Sun Jan 01 00:00:00 EST 1989

OSTI ID:6699934

Myers, R B

Initial studies demonstrated that treatment of male rats with estradiol for a period of four days resulted in a reduction in {sup 3}H-thymidine incorporation of isolated interstitial cells. Furthermore, {sup 3}H-thymidine incorporation in interstitial cells of 33 day old rats was inhibited by the addition of estradiol in vitro. Subsequent studies were performed in the ethylene dimethanesulphonate (EDS) treated rat. Leydig cells were rapidly destroyed after EDS administration as determined by hCG binding, steroid synthesis and morphological studies. A significant finding was the production of 5{alpha}-androstane-3{alpha},17{beta}-diol by regeneration Leydig cells of the EDS treated rat. In subsequent studies, rats received daily treatment with estradiol and/or hCG/LH after EDS treatment. Estradiol treatment had no effect on Leydig cell degeneration. Leydig cell regeneration, however, did not occur in the estradiol treated rat.

• Research Organization: Medical Coll. of Georgia, Augusta, GA (USA)
• OSTI ID: 6699934
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CELL DIFFERENTIATION
ESTRADIOL
BIOCHEMICAL REACTION KINETICS
RATS
SULFONIC ACID ESTERS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
AZINES
ESTERS
ESTRANES
ESTROGENS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
STEROID HORMONES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques