Detection of antibody against antigen expressed by molecularly cloned hepatitis C virus cDNA: Application to diagnosis and blood screening for posttransfusion hepatitis

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.3.983· OSTI ID:6699784

Miyamura, Tatsuo; Saito, Izumu ^[1]; Katayama, Tohru ^[2]; Kikuchi, Shu; Tateda, Akira ^[3]; Houghton, M; Choo, Quilim; Kuo, G ^[4]

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1. National Institute of Health, Tokyo (Japan)
2. Tokyo National Chest Hospital (Japan)
3. Sendai National Hospital (Japan)
4. Chiron Corporation, Emeryville, CA (USA)

A cDNA clone has been derived from the plasma of a chimpanzee with chronic non-A, non-B viral hepatits (NANBH). The authors have assayed for antibodies reacting with the encoded antigen in sera from posttransfusion hepatitis patients (643 samples from 23 patients) and their corresponding donors collected during the past 10 years in Japan. The antibody was detected in 15 out of 17 (88.2%) posttransfusion NANBH (PT-NANBH) patients whose sera over time displayed multiple alanine aminotransferase (ALT) peaks. In general, the antibody was detected after several peaks of serum ALT elevations and, once detected, it persisted for years. Of the 15 well-defined cases of PT-NANBH that showed multiple ALT peaks and hepatitis C virus seroconversions, 11 (73.3%) were shown to be transfused with at least one unit of blood positive for the antibody. The retrospective analysis showed that all tested donor blood found to be positive for the antibody had been transfused to recipients who afterwards developed NANBH. These data strongly suggest that the cloned cDNA originated from an etiological agent of NANBH termed the hepatitis C virus. Furthermore, the present study demonstrates that had the screening been done with the anti-hepatitis C virus assay, 11 out of 17 (64.7%) cases of chronic PT-NANBH and 1 out of 6 (16.6%) acute PT-NANBH would have been prevented.

• OSTI ID: 6699784
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:3; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
RADIOIMMUNOASSAY
HEPATITIS
ETIOLOGY
RECOMBINANT DNA
DNA-CLONING
ABSCESSES
AMINOTRANSFERASES
ANTIGENS
FISTULAE
IODINE 125
JAPAN
NEOPLASMS
PATIENTS
PRIMATES
TRANSFUSIONS
VIRUSES
ANIMALS
ASIA
BETA DECAY RADIOISOTOPES
BIOASSAY
CLONING
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM DISEASES
DISEASES
DNA
DNA HYBRIDIZATION
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HYBRIDIZATION
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICROORGANISMS
NITROGEN TRANSFERASES
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PATHOLOGICAL CHANGES
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
THERAPY
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques