Vanadate-induced inhibition of renin secretion is unrelated to inhibition Na,K-ATPase activity

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90511-O· OSTI ID:6695454

Churchill, P C; Rossi, N F; Churchill, M C; Ellis, V R ^[1]

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1. Wayne State Univ. School of Medicine, Detroit, MI (USA)

There is evidence that three inhibitors of Na,K-ATPase activity-ouabain, K-free extracellular fluid, and vanadate--inhibit renin secretion by increasing Ca{sup 2+} concentration in juxtaglomerular cells, but in the case of vanadate, it is uncertain whether the increase in Ca{sup 2+} is due to a decrease in Ca{sup 2+} efflux or to an increase in Ca{sup 2+} influx through potential operated Ca channels. In the present experiments, the rat renal cortical slice preparation was used to compare and contrast the effects of ouabain, of K-free fluid, and of vanadate on renin secretion, in the absence and presence of methoxyverapamil, A Ca channel blocker. Basal renin secretory rate averaged 7.7 {plus minus} 0.3 GU/g/60 min, and secretory rate was reduced to nearly zero by 1 mM ouabain, by K-free fluid, by 0.5 mM vanadate, and by K-depolarization. Although 0.5 {mu}M methoxyverapamil completely blocked the inhibitory effect of K-depolarization, it failed to antagonize the inhibitory effects of ouabain, of K-free fluid, and of vanadate.

• OSTI ID: 6695454
• Journal Information: Life Sciences; (USA), Vol. 46:26; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ATP-ASE
ENZYME ACTIVITY
CALCIUM COMPOUNDS
MEMBRANE TRANSPORT
RENIN
SECRETION
VANADIUM
BIOLOGICAL EFFECTS
ENZYME INHIBITORS
INHIBITION
OUABAIN
PORINS
ACID ANHYDRASES
ALKALINE EARTH METAL COMPOUNDS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DRUGS
ELEMENTS
ENZYMES
GLYCOSIDES
HYDROLASES
MEMBRANE PROTEINS
METALS
NONSPECIFIC PEPTIDASES
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PHOSPHOHYDROLASES
PROTEINS
STROPHANTHINS
TRANSITION ELEMENTS
560300* - Chemicals Metabolism & Toxicology