Effects of acetylcholine and other agents on /sup 32/P-prelabeled phosphoinositides and phosphatidate in crude synaptosomal preparations
Experimental conditions are described which permit effects of various agents on polyphosphoinositides and phosphatidic acid (PA) to be evaluated simultaneously in crude nerve-ending preparations from rat brain. Acetylcholine (3-100 microM) or carbachol (30-1,000 microM) induced the hydrolysis of prelabeled polyphosphoinositides and, at the same time, stimulated the net label incorporated in phosphatidic acid. All muscarinic effects were blocked by atropine or pirenzepine. Non-muscarinic agonists (glutamate, adenosine, norepinephrine) stimulated polyphosphoinositide hydrolysis in this preparation, but of these only norepinephrine affected phosphatidic acid turnover. A potentiation of acetylcholine-induced phosphoinositide turnover by KCl was observed, as well as an apparent selective inhibition of PIP2 hydrolysis by LiCl. Acetylcholine-stimulated turnover of PA was not necessarily coupled to phosphoinositide hydrolysis.
- Research Organization:
- Wellcome Research Laboratories, Research Triangle Park, NC (USA)
- OSTI ID:
- 6691737
- Journal Information:
- J. Neurosci. Res.; (United States), Vol. 20:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACETYLCHOLINE
BIOCHEMICAL REACTION KINETICS
PARASYMPATHOMIMETICS
PHOSPHOLIPIDS
METABOLISM
ATROPINE
BRAIN
CALCIUM
CHLORIDES
LITHIUM
NERVE CELLS
PHOSPHATES
PHOSPHORUS 32
RATS
TRACER TECHNIQUES
ALKALI METALS
ALKALINE EARTH METALS
ALKALOIDS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CENTRAL NERVOUS SYSTEM
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
ESTERS
HALIDES
HALOGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPIDS
MAMMALS
METALS
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PARASYMPATHOLYTICS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
QUATERNARY COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques