2,4,5,2',4',5'-Hexachlorobiphenyl-lipoprotein (LDL, HDL, VLDL) interaction and induced lipidosis in cultured skin fibroblasts

Journal Article · Fri Jun 01 00:00:00 EDT 1984 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(84)90078-1· OSTI ID:6678132

Kling, D; Becker, M M; Kruth, H S; Gamble, W

2,4,5,2',4',5'-Hexachlorobiphenyl (HCB) induced cytoplasmic inclusions and lipidosis in normal (AG1437) and hypercholesterolemic (GM488) human skin fibroblasts. Quantitative and qualitative microscopic fluorescence analysis showed that the cytoplasmic inclusions are formed as early as 3 hr after treatment with HCB. The inclusions contain lipids but no detectable nonesterified cholesterol or cholesteryl ester. High density lipoproteins (HDL), low density lipoproteins (LDL), and very low density lipoproteins (VLDL) facilitate the apparent uptake of HCB by skin fibroblasts. HDL and LDL appeared to reverse the induction of cytoplasmic inclusions and lipidosis when cells were pretreated with HCB, the HCB was removed from media, and the cells were incubated with LDL or HDL. The results suggest that lipoproteins participate in the uptake and egress of HCB from skin fibroblasts.

• Research Organization: Oregon State Univ., Corvallis
• OSTI ID: 6678132
• Journal Information: Environ. Res.; (United States), Vol. 34:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHLORINATED AROMATIC HYDROCARBONS
BIOLOGICAL EFFECTS
UPTAKE
FIBROBLASTS
ULTRASTRUCTURAL CHANGES
LIPOPROTEINS
BIOCHEMICAL REACTION KINETICS
CELL CULTURES
MAN
MEMBRANE TRANSPORT
SKIN
ANIMAL CELLS
ANIMALS
AROMATICS
BODY
CONNECTIVE TISSUE CELLS
HALOGENATED AROMATIC HYDROCARBONS
KINETICS
LIPIDS
MAMMALS
MORPHOLOGICAL CHANGES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
PRIMATES
PROTEINS
REACTION KINETICS
SOMATIC CELLS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)