Antisense RNA suppression of peroxidase gene expression

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6668693

Lagrimini, L M; Bradford, S; De Leon, F D ^[1]

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1. Ohio State Univ., Columbus (USA)

The 5{prime} half the anionic peroxidase cDNA of tobacco was inserted into a CaMV 35S promoter/terminator expression cassette in the antisense configuration. This was inserted into the Agrobacterium-mediated plant transformation vector pCIBIO which includes kanamycin selection, transformed into two species of tobacco (N. tabacum and M. sylvestris), and plants were subsequently regenerated on kanamycin. Transgenic plants were analyzed for peroxidase expression and found to have 3-5 fold lower levels of peroxidase than wild-type plants. Isoelectric focusing demonstrated that the antisense RNA only suppressed the anionic peroxidase. Wound-induced peroxidase expression was found not to be affected by the antisense RNA. Northern blots show a greater than 5 fold suppression of anionic peroxidase mRNA in leaf tissue, and the antisense RNA was expressed at a level 2 fold over the endogenous mRNA. Plants were self-pollinated and F1 plants showed normal segregation. N. sylvestris transgenic plants with the lowest level of peroxidase are epinastic, and preliminary results indicate elevated auxin levels. Excised pith tissue from both species of transgenic plants rapidly collapse when exposed to air, while pith tissue from wild-type plants showed little change when exposed to air. Further characterization of these phenotypes is currently being made.

• OSTI ID: 6668693
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
NICOTIANA
GENE REGULATION
PEROXIDASES
GENES
AUXINS
BACTERIA
MESSENGER-RNA
PHENOTYPE
ENZYMES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PLANT GROWTH REGULATORS
PLANTS
RNA
140505* - Solar Energy Conversion- Photochemical
Photobiological
& Thermochemical Conversion- (1980-)