Effect of laser microbeam irradiation of the nucleus on the cleavage of mouse eggs in culture
Two-cell mouse eggs were irradiated by a helium-cadmium laser on a spot of about 4 ..mu..m/sup 2/ in one or both nuclei either continuously or repeatedly at 0.36 erg ..mu..m/sup -2/ sec/sup -1/ and then cultured to observe cellular development. After exposing one nucleus to the microbeam to five or seven 1-sec pulses, about 45% developed to the 3-cell stage in 24 hr of culture. In overnight cultures of the 2-cell eggs in which both nuclei were irradiated for 9 or 20 sec continuously, 40 (9 sec) and 50% (20 sec) of the eggs remained at the 2-cell stage, while 45 (9 sec) and 25% (20 sec) developed to the 4-cell stage. When both nuclei were each irradiated by a 9-sec continuous laser beam (totaling 13 ergs), about 40% of the embryos of the 2-cell stage did not divide. The effect of seven pulses on the blastomere cleavage of 2-cell mouse eggs appeared to be comparable to that of continuous 9-sec laser irradiation. Both pulse and continuous laser microirradiation methods may be developed for inactivation of the nucleus as a nonpipetting, less injurious method for enucleation of mammalian eggs.
- Research Organization:
- Univ. of California, San Francisco
- DOE Contract Number:
- AM03-76SF00034
- OSTI ID:
- 6665420
- Journal Information:
- Radiat. Res.; (United States), Vol. 98:3
- Country of Publication:
- United States
- Language:
- English
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CELL NUCLEI
INACTIVATION
EGGS
CELL DIVISION
LASER RADIATION
BIOLOGICAL EFFECTS
GAS LASERS
MICE
ANIMALS
CELL CONSTITUENTS
ELECTROMAGNETIC RADIATION
LASERS
MAMMALS
RADIATIONS
RODENTS
VERTEBRATES
560400* - Other Environmental Pollutant Effects