Chimeric proteins define variable and essential regions of Ha-ras-encoded protein
The biological role of amino acid differences between the human 21-kDa Ha-ras protein (p21) and the human 23-kDa R-ras protein (p23) was investigated by engineering mutant Ha-ras p21 molecules containing divergent amino acid sequences from R-ras p23. Variant amino acids from R-ras p23 regions 1-30, 52-57, 67-78, 1-30 and 67-78 together, and 112-124 were substituted for the corresponding Ha-ras p21 amino acid regions 1-4, 26-31, 41-52, 1-4 and 41-52 together, and 86-98, respectively. Rat fibroblasts transfected with genes encoding these position-12 valine-substituted chimeric Ha-ras proteins displayed the same properties of morphological transformation and anchorage-independent growth as Ha-ras T24 oncogene-transformed fibroblasts. However, substitution of variant amino acids from the 80 C-terminal residues (amino acids 138-218) of R-ras p23 for the corresponding p21 amino acids (residues 112-189) inactivated the transforming activity of position-12 valine-substituted p21. The converse substitution of Ha-ras p21 C-terminal residues into R-ras p23 did not result in transformation by position-38 valine-substituted p232. These data are discussed in terms of the structure of ras proteins and the nature of interactions determining the specificity of effector function.
- Research Organization:
- Genentech, Inc., San Francisco, CA (USA)
- OSTI ID:
- 6659401
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Identification of an intracellular protein that specifically interacts with photoaffinity-labeled oncogenic p21 protein
Characterization of cDNA encoding mouse DNA repair protein O sup 6 -methylguanine-DNA methyltransferase and high-level expression of the wild-type and mutant proteins in Escherichia coli
Related Subjects
FIBROBLASTS
MORPHOLOGICAL CHANGES
ONCOGENES
TRANSCRIPTION
PROTEINS
AMINO ACID SEQUENCE
CHIMERAS
ELECTROPHORESIS
METHIONINE
MUTANTS
ONCOGENIC TRANSFORMATIONS
RATS
SULFUR 35
VALINE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
GENES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MOLECULAR STRUCTURE
MOSAICISM
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SULFUR ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques