Vertical integration of cosmid and YAC resources for interval mapping on the X-chromosome

Journal Article · Mon Feb 01 00:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1060· OSTI ID:6653210

Holland, J; Coffey, A J; Giannelli, F; Bentley, D R ^[1]

---

1. United Medical and Dental Schools of Guy's and St. Thomas's Hospitals, London (United Kingdom)

The vertical integration of cosmid and yeast artificial chromosome (YAC) resources is of particular importance in the development of high-resolution maps of selected regions of the human genome. A resource of approximately 95,000 cosmids constructed using DNA from primary fibroblasts of karyotype 49,XXXXX was validated by detailed characterization of a 200-kb cosmid contig spanning exons 8-20 of the dystrophin gene. This resource was used to construct contigs in 0.65 Mb of Xq26 by hybridization of gel-purified YAC DNA to high-density gridded arrays of the cosmid library; positive cosmids were overlapped by fingerprinting. Contigs were oriented and ordered relative to existing YACs in the region using cross-hybridization. The overlaps between a representative set of cosmids define 54 intervals of 5-20 kb and were used to construct a high-resolution cosmid interval map of the region, locating markers, dinucleotide repeats, and candidate CpG islands. This approach can be applied rapidly to large regions of the genome and without recourse to subcloning of individual YACs. 49 refs., 5 figs.

• OSTI ID: 6653210
• Journal Information: Genomics; (United States), Vol. 15:2; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HUMAN CHROMOSOMES
GENETIC MAPPING
HUMAN X CHROMOSOME
COSMIDS
DNA
DNA SEQUENCING
FIBROBLASTS
GENOME MUTATIONS
HYBRIDIZATION
KARYOTYPE
MAPS
ANIMAL CELLS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
HETEROCHROMOSOMES
MAPPING
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
X CHROMOSOME
550400* - Genetics