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Title: Human bradykinin B2 receptor: Nucleotide sequence analysis and assignment to chromosome 14

Abstract

Functional cDNA clones for human bradykinin B2 receptor were isolated from uterus RNA by a polymerase chain reaction (PCR)-based method and by screening a human cosmid library with rat bradykinin B2 receptor probe. We isolated several overlapping clones from the cosmid library, each of which encodes the entire protein-coding sequence. The human bradykinin B2 receptor gene codes for a 364-amino-acid protein with a molecular mass of 41,442 Da that is highly homologous to rat bradykinin B2 receptor cDNA (81%). The entire human cDNA sequence was cloned into an expression vector and mRNA was synthesised by in vitro transcription. Applications of bradykinin caused membrane current responses in Xenopus oocytes injected with the in vitro-synthesized mRNA. Preincubation with the potent B2 antagonist, HOE140, prevented this response. The genomic clone is intronless, and we have identified an upstream promoter region and a downstream polyadenylation signal. The human bradykinin B2 receptor gene has been mapped to chromosome 14 using PCR to specifically amplify DNA from somatic cell hybrids. 10 refs., 1 fig., 1 tab.

Authors:
; ; ; ; ;  [1]
  1. ICI Pharmaceuticals, Cheshire (United Kingdom)
Publication Date:
OSTI Identifier:
6652873
Resource Type:
Journal Article
Journal Name:
Genomics; (United States)
Additional Journal Information:
Journal Volume: 15:2; Journal ID: ISSN 0888-7543
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BRADYKININ; RECEPTORS; HUMAN CHROMOSOME 14; GENETIC MAPPING; DNA SEQUENCING; COSMIDS; DNA; DNA-CLONING; GENE AMPLIFICATION; LIBRARIES; OOCYTES; RNA; SCREENING; TRANSCRIPTION; UTERUS; BODY; CHROMOSOMES; CLONING; DNA HYBRIDIZATION; FEMALE GENITALS; GERM CELLS; HUMAN CHROMOSOMES; HYBRIDIZATION; KININS; MAPPING; MEMBRANE PROTEINS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PEPTIDES; POLYPEPTIDES; PROTEINS; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics

Citation Formats

Powell, S J, Slynn, G, Thomas, C, Hopkins, B, Briggs, I, and Graham, A. Human bradykinin B2 receptor: Nucleotide sequence analysis and assignment to chromosome 14. United States: N. p., 1993. Web. doi:10.1006/geno.1993.1084.
Powell, S J, Slynn, G, Thomas, C, Hopkins, B, Briggs, I, & Graham, A. Human bradykinin B2 receptor: Nucleotide sequence analysis and assignment to chromosome 14. United States. https://doi.org/10.1006/geno.1993.1084
Powell, S J, Slynn, G, Thomas, C, Hopkins, B, Briggs, I, and Graham, A. 1993. "Human bradykinin B2 receptor: Nucleotide sequence analysis and assignment to chromosome 14". United States. https://doi.org/10.1006/geno.1993.1084.
@article{osti_6652873,
title = {Human bradykinin B2 receptor: Nucleotide sequence analysis and assignment to chromosome 14},
author = {Powell, S J and Slynn, G and Thomas, C and Hopkins, B and Briggs, I and Graham, A},
abstractNote = {Functional cDNA clones for human bradykinin B2 receptor were isolated from uterus RNA by a polymerase chain reaction (PCR)-based method and by screening a human cosmid library with rat bradykinin B2 receptor probe. We isolated several overlapping clones from the cosmid library, each of which encodes the entire protein-coding sequence. The human bradykinin B2 receptor gene codes for a 364-amino-acid protein with a molecular mass of 41,442 Da that is highly homologous to rat bradykinin B2 receptor cDNA (81%). The entire human cDNA sequence was cloned into an expression vector and mRNA was synthesised by in vitro transcription. Applications of bradykinin caused membrane current responses in Xenopus oocytes injected with the in vitro-synthesized mRNA. Preincubation with the potent B2 antagonist, HOE140, prevented this response. The genomic clone is intronless, and we have identified an upstream promoter region and a downstream polyadenylation signal. The human bradykinin B2 receptor gene has been mapped to chromosome 14 using PCR to specifically amplify DNA from somatic cell hybrids. 10 refs., 1 fig., 1 tab.},
doi = {10.1006/geno.1993.1084},
url = {https://www.osti.gov/biblio/6652873}, journal = {Genomics; (United States)},
issn = {0888-7543},
number = ,
volume = 15:2,
place = {United States},
year = {Mon Feb 01 00:00:00 EST 1993},
month = {Mon Feb 01 00:00:00 EST 1993}
}