Nuclear proteins interacting with the promoter region of the human granulocyte/macrophage colony-stimulating factor gene
The gene for human granulocyte/macrophage colony-stimulating factor (GM-CSF) is expressed in a tissue-specific as well as an activation-dependent manner. The interaction of nuclear proteins with the promoter region of the GM-CSF gene that is likely to be responsible for this pattern of GM-CSF expression was investigated. The authors show that nuclear proteins interact with DNA fragments from the GM-CSF promoter in a cell-specific manner. A region spanning two cytokine-specific sequences, cytokine 1 (CK-1, 5', GAGATTCCAC 3') and cytokine 2 (CK-2, 5' TCAGGTA 3') bound two nuclear proteins from GM-CSF-expressing cells in gel retardation assays. NF-GMb was inducible with phorbol 12-myristate 13-acetate and accompanied induction of GM-CSF message. NF-GMb was absent in cell lines not producing GM-CSF, some of which had other distinct binding proteins. NF-GMa and NF-GMb eluted from a heparin-Sepharose column at 0.3 and 0.6 M KCl, respectively. They hypothesize that the sequences CK-1 and CK-2 bind specific proteins and regulate GM-CSF transcription.
- Research Organization:
- Institute of Medical and Veterinary Science, Adelaide (Australia)
- OSTI ID:
- 6652300
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:3
- Country of Publication:
- United States
- Language:
- English
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GENE REPRESSORS
DNA SEQUENCING
GROWTH FACTORS
GENE REGULATION
PHORBOL ESTERS
BIOLOGICAL EFFECTS
ATP
BLADDER
CARCINOMAS
GENES
GLUCOPROTEINS
LEUKOCYTES
MACROPHAGES
MAN
MELANOMAS
PHOSPHORUS 32
TRANSCRIPTION
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ESTERS
GLYCOPROTEINS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
MITOGENS
NEOPLASMS
NUCLEI
NUCLEOPROTEINS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
URINARY TRACT
VERTEBRATES
550201* - Biochemistry- Tracer Techniques