Characterization of in vitro translation products
Journal Article
·
· Methods Enzymol.; (United States)
This chapter describes the characterization of in vitro translation products by the most commonly used techniques. The methods include SDS-polyacrylamide gel electrophoresis (SDS-PAGE), combined with immunoprecipitation and/or fluorography of (/sup 35/S)methionine-labeled translation products. The other frequently used characterization tool, translation of hybrid-selected mRNA or hybrid-arrested translation, is treated separately in this volume. Methods are also given for the recognition of mRNAs coding for secreted or membrane proteins.
- OSTI ID:
- 6651794
- Journal Information:
- Methods Enzymol.; (United States), Vol. 152
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Conference
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· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
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OSTI ID:6651794
Electrophoretic separation of in vitro translation products on giant two-dimensional gels allows detailed analysis of cellular mRNAs
Journal Article
·
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Journal Article
·
Wed Jul 16 00:00:00 EDT 1986
· Biochem. Biophys. Res. Commun.; (United States)
·
OSTI ID:6651794
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
MESSENGER-RNA
AUTORADIOGRAPHY
PROTEINS
ELECTROPHORESIS
CARBON 14 COMPOUNDS
IN VITRO
METHIONINE
MOLECULAR BIOLOGY
PRECIPITATION
RECOMBINANT DNA
SULFUR 35
TRITIUM COMPOUNDS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
EVEN-ODD NUCLEI
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
RNA
SEPARATION PROCESSES
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques
MESSENGER-RNA
AUTORADIOGRAPHY
PROTEINS
ELECTROPHORESIS
CARBON 14 COMPOUNDS
IN VITRO
METHIONINE
MOLECULAR BIOLOGY
PRECIPITATION
RECOMBINANT DNA
SULFUR 35
TRITIUM COMPOUNDS
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
EVEN-ODD NUCLEI
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
RNA
SEPARATION PROCESSES
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques