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Title: Small-scale field test of the genetically engineered lacZY marker

Abstract

Commercial genetic engineering is advancing into areas that require the small-scale introduction of genetically engineered microorganisms (GEMs) to better quantify variables that affect microorganism distribution and survival and to document potential long-term consequences. A recombinant DNA marker system, the lacZY marker, developed by the Monsanto Agricultural Co., enables the distribution and fate of marked fluorescent pseudomonad organisms to be monitored under actual field conditions. Critical evaluation of GEMs under field conditions is imperative if plant-beneficial effects are to be correlated with organism release. This paper evaluates the effectiveness of this marker system and its ability to facilitate the assessment of risks associated with deliberate environmental introductions of genetically engineered microorganisms. Results of prerelease contained growth chamber and field experiments demonstrated that: (1) the scientific risk assessment methodology adopted by Monsanto and approved by the U.S. Environmental Protection Agency was appropriate and comprehensive; (2) the deliberate introduction of a GEM did not pose unacceptable or unforeseen risks to human health or the environment; (3) the lacZY marker is an effective environmental tracking tool; and (4) regulatory oversight should reflect the expected risk and not be excessively burdensome for all GEMs.

Authors:
; ;  [1]
  1. Oak Ridge National Laboratory, TN (USA)
Publication Date:
OSTI Identifier:
6651579
Resource Type:
Journal Article
Journal Name:
Regulatory Toxicology and Pharmacology; (USA)
Additional Journal Information:
Journal Volume: 11:3; Journal ID: ISSN 0273-2300
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENETIC ENGINEERING; BIOLOGICAL MARKERS; GENE OPERONS; PSEUDOMONAS; RECOMBINANT DNA; RISK ASSESSMENT; BACTERIA; DNA; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; 550200* - Biochemistry

Citation Formats

Hattemer-Frey, H A, Brandt, E J, and Travis, C C. Small-scale field test of the genetically engineered lacZY marker. United States: N. p., 1990. Web. doi:10.1016/0273-2300(90)90025-7.
Hattemer-Frey, H A, Brandt, E J, & Travis, C C. Small-scale field test of the genetically engineered lacZY marker. United States. https://doi.org/10.1016/0273-2300(90)90025-7
Hattemer-Frey, H A, Brandt, E J, and Travis, C C. 1990. "Small-scale field test of the genetically engineered lacZY marker". United States. https://doi.org/10.1016/0273-2300(90)90025-7.
@article{osti_6651579,
title = {Small-scale field test of the genetically engineered lacZY marker},
author = {Hattemer-Frey, H A and Brandt, E J and Travis, C C},
abstractNote = {Commercial genetic engineering is advancing into areas that require the small-scale introduction of genetically engineered microorganisms (GEMs) to better quantify variables that affect microorganism distribution and survival and to document potential long-term consequences. A recombinant DNA marker system, the lacZY marker, developed by the Monsanto Agricultural Co., enables the distribution and fate of marked fluorescent pseudomonad organisms to be monitored under actual field conditions. Critical evaluation of GEMs under field conditions is imperative if plant-beneficial effects are to be correlated with organism release. This paper evaluates the effectiveness of this marker system and its ability to facilitate the assessment of risks associated with deliberate environmental introductions of genetically engineered microorganisms. Results of prerelease contained growth chamber and field experiments demonstrated that: (1) the scientific risk assessment methodology adopted by Monsanto and approved by the U.S. Environmental Protection Agency was appropriate and comprehensive; (2) the deliberate introduction of a GEM did not pose unacceptable or unforeseen risks to human health or the environment; (3) the lacZY marker is an effective environmental tracking tool; and (4) regulatory oversight should reflect the expected risk and not be excessively burdensome for all GEMs.},
doi = {10.1016/0273-2300(90)90025-7},
url = {https://www.osti.gov/biblio/6651579}, journal = {Regulatory Toxicology and Pharmacology; (USA)},
issn = {0273-2300},
number = ,
volume = 11:3,
place = {United States},
year = {Fri Jun 01 00:00:00 EDT 1990},
month = {Fri Jun 01 00:00:00 EDT 1990}
}