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Title: DNA sequencing: chemical methods

Abstract

Limited base-specific or base-selective cleavage of a defined DNA fragment yields polynucleotide products, the length of which correlates with the positions of the particular base (or bases) in the original fragment. Sverdlov and co-workers recognized the possibility of using this principle for the determination of DNA sequences. In 1977 a fully elaborated method was introduced based on this principle, which allowed routine analysis of DNA sequences over distances greater than 100 nucleotide unite from a defined, radiolabeled terminus. Six procedures for partial cleavage were described. Simultaneous parallel resolution of an appropriate set of partial cleavage mixtures by polyacrylamide gel electrophoresis, followed by visualization of the radioactive bands by autoradiography, allows the deduction of nucleotide sequence.

Authors:
;
Publication Date:
OSTI Identifier:
6650885
Resource Type:
Journal Article
Journal Name:
Methods Enzymol.; (United States)
Additional Journal Information:
Journal Volume: 152
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; DNA; AUTORADIOGRAPHY; DNA SEQUENCING; MOLECULAR BIOLOGY; CHROMOSOMES; ELECTROPHORESIS; HYBRIDIZATION; RECOMBINANT DNA; RNA; NUCLEIC ACIDS; ORGANIC COMPOUNDS; STRUCTURAL CHEMICAL ANALYSIS; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Ambrose, B J.B., and Pless, R C. DNA sequencing: chemical methods. United States: N. p., 1987. Web. doi:10.1016/0076-6879(87)52059-6.
Ambrose, B J.B., & Pless, R C. DNA sequencing: chemical methods. United States. https://doi.org/10.1016/0076-6879(87)52059-6
Ambrose, B J.B., and Pless, R C. 1987. "DNA sequencing: chemical methods". United States. https://doi.org/10.1016/0076-6879(87)52059-6.
@article{osti_6650885,
title = {DNA sequencing: chemical methods},
author = {Ambrose, B J.B. and Pless, R C},
abstractNote = {Limited base-specific or base-selective cleavage of a defined DNA fragment yields polynucleotide products, the length of which correlates with the positions of the particular base (or bases) in the original fragment. Sverdlov and co-workers recognized the possibility of using this principle for the determination of DNA sequences. In 1977 a fully elaborated method was introduced based on this principle, which allowed routine analysis of DNA sequences over distances greater than 100 nucleotide unite from a defined, radiolabeled terminus. Six procedures for partial cleavage were described. Simultaneous parallel resolution of an appropriate set of partial cleavage mixtures by polyacrylamide gel electrophoresis, followed by visualization of the radioactive bands by autoradiography, allows the deduction of nucleotide sequence.},
doi = {10.1016/0076-6879(87)52059-6},
url = {https://www.osti.gov/biblio/6650885}, journal = {Methods Enzymol.; (United States)},
number = ,
volume = 152,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1987},
month = {Thu Jan 01 00:00:00 EST 1987}
}