Induced DNA repair pathway in mammalian cells
The survival of cultured rat kangaroo cells (PtK-2) and human xeroderma pigmentosum cells incubated with 5 ..mu..M cycloheximide subsequent to ultraviolet irradiation is lower than that of cells incubated without cycloheximide. The drop in survival is considerably larger than that produced by incubation of unirradiated cells with cycloheximide. The phenomenon was also observed when PtK-2 cells were incubated with emetine, another protein synthesis inhibitor, or with 5,6-dichloro-1-..beta..-D-ribofuranosylbenzimidazole, a RNA synthesis inhibitor. PtK cells which received a preliminary UV treatment followed by an incubation period without cycloheximide and then a second irradiation and 24 hour incubation with cycloheximide, survived the effects of the second irradiation better than cells which were incubated in the presence of cycloheximide after the first and second UV irradiation. The application of cycloheximide for 24 hours after UV irradiation of PtK cells resulted in one-half as many 6-thioguanine resistant cells as compared to the number of 6-thioguanine resistant cells found when cycloheximide was not used. These experiments indicate that a UV-inducible cycloheximide-sensitive DNA repair pathway is present in PtK and xeroderma pigmentosum cells, which is error-prone in PtK cells.
- Research Organization:
- Texas Univ., Dallas (USA)
- OSTI ID:
- 6646187
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL CULTURES
SURVIVAL TIME
CYCLOHEXIMIDE
BIOLOGICAL EFFECTS
DNA
BIOLOGICAL REPAIR
RNA
BIOSYNTHESIS
ULTRAVIOLET RADIATION
MAMMALS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL RECOVERY
DRUGS
ELECTROMAGNETIC RADIATION
FUNGICIDES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PESTICIDES
RADIATIONS
RECOVERY
REPAIR
SYNTHESIS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture