A V1-vascular vasopressin antagonist suitable for radioiodination and photoaffinity labeling

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · American Journal of Hypertension; (USA)

DOI:https://doi.org/10.1093/ajh/3.6.471· OSTI ID:6644075

Thibonnier, M; Chehade, N; Hinko, A ^[1]

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1. University Hospital of Cleveland, O (USA)

We have previously characterized the V1-vascular arginine vasopressin (AVP) receptors of human platelets. We now report on a radiomonoiodinated and photoreactive V1-vascular AVP antagonist (V1-ag) to be used for the purification of human V1-vascular AVP receptors. The V1-ag, d(CH2)5Tyr(Me)Tyr(NH2)AVP was modified by radiomonoiodination of d(CH2)5-Tyr(Me)Tyr(NH2)AVP with the Iodogen technique, and derivatization of d(CH2)5Tyr(Me)Tyr(125I)(NH2)-AVP with the photoreactive crosslinker, N-hydroxysuccinimidyl-4-azidobenzoate (HSAB) (each step included HPLC purification). In competition experiments, the affinity of these V1-ag for the human platelet AVP receptors remained excellent. Irreversible photoaffinity labeling of the platelet V1-vascular AVP receptor was successfully achieved by UV lamp exposure (365 nm, 20 min). Thus, AzBz-d(CH2)5Tyr(Me)Tyr(125I)(NH2)AVP is a promising tool to use for the purification of human V1-vascular AVP receptors.

• OSTI ID: 6644075
• Journal Information: American Journal of Hypertension; (USA), Vol. 3; ISSN 0895-7061
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PARASYMPATHOLYTICS
CHEMICAL PREPARATION
RECEPTORS
FRACTIONATION
VASOPRESSIN
AFFINITY
BLOOD PLATELETS
CELL MEMBRANES
IODINATION
IODINE 125
LIQUID COLUMN CHROMATOGRAPHY
MAN
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HALOGENATION
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques