Influences of polar auxin transport on polarity of adventitious bud formation in hybrid populas
- Yonsei Univ. Kangwondo (Korea)
- Univ of Minnesota, St. Paul (USA)
The role of auxin and cytokinin distribution of polar regeneration of adventitious bud has been investigated. Explants from leaf midvein were labelled with {sup 14}C-NAA and {sup 14}C-BA and the radioactivity in proximal, mid, and distal portions was counted after 24h and 48h. Explants showing polar regeneration of buds on the proximal end showed a clear polar distribution of {sup 14}CNAA. Auxin transport inhibitors (NPA, TIBA) eliminated polar distribution of auxin and reduced polarity of bud formation and the total number of buds formed, but did not reduce callus formation. Increased concentration of Ca(NO{sub 3}){sub 2} decreased polarity of bud formation and increased the number of buds formed but did not affect the distribution of auxin of cytokinin. Some factor in addition to polar distribution of auxin or cytokinin-auxin ratio appears to influence the polarity of adventitious bud formation.
- OSTI ID:
- 6643729
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AUXINS
BIOLOGICAL FUNCTIONS
BUDS
BIOLOGICAL REGENERATION
KININS
CARBON 14 COMPOUNDS
MEMBRANE TRANSPORT
POPLARS
TRACER TECHNIQUES
BIOLOGICAL RECOVERY
FUNCTIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC COMPOUNDS
PEPTIDES
PLANT GROWTH REGULATORS
PLANTS
POLYPEPTIDES
PROTEINS
RECOVERY
TREES
550201* - Biochemistry- Tracer Techniques