Incorporation of monomethylethanolamine into phosphatidylcholine by way of an exchange reaction followed by methylation
- Louisiana State Univ., Baton Rouge (USA)
Recent evidence by Datko and Mudd indicates that phosphatidylcholine (PC) may be synthesized by methylation of phosphatidylmonomethyl-ethanolamine (PMME), but perhaps not by utilization of phosphatidylethanolamine (PE) as a source of PMME. They provided evidence that a CDP derivative of monomethylethanolamine (MME) might be the source of the headgroup. Another possibility is incorporation of MME by an exchange reaction. We tested this by incubating MME with ER from castor bean endosperm and radiolabeled S- adenosylmethionine under conditions which would allow incorporation of the headgroup and methylation to PC. Under these conditions the reaction proceeded, with radiolabel appearing in both PC and phosphatidyldimethylethanolamine. Neither ethanolamine nor L-serine, both of which are known to undergo exchange reactions, yielded PC under the same conditions.
- OSTI ID:
- 6643626
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
Similar Records
Phosphatidylcholine synthesis in castor bean endosperm. Metabolism of S-adenosylmethionine and ethanolamine
Do cholinephosphotransferase and phosphatidylethanolamine methyltransferase synthesize different species of phosphatidylcholine
Related Subjects
AMINES
METHYLATION
PHOSPHOLIPIDS
BIOSYNTHESIS
CASTOR
METHIONINE
RADIOISOTOPES
TRACER TECHNIQUES
AMINO ACIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DRUGS
ESTERS
EUPHORBIA
ISOTOPE APPLICATIONS
ISOTOPES
LIPIDS
LIPOTROPIC FACTORS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MEDICINAL PLANTS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques