Phosphoinositolphosphate (PIP) cascade induction by hypertonic stress of plant tissue

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6643262

Srivastava, A; Jacoby, B ^[1]

---

1. Hebrew Univ. of Jerusalem, Rehovot (Israel)

Inositol 1,4,5-trisphosphate (IP{sub 3}) was determined by competition with ({sup 3}H)-IP{sub 3} for binding to an IP{sub 3} specific protein. A hypertonic mannitol, sorbitol or lactose shock induced an increase in the rate of K{sup +} uptake and raised the IP{sub 3} content of Beta vulgaris slices, excised Vigna mungo and Sorghum bicolor roots, as well as attached V. mungo roots. Increased K{sup +} uptake could also be induced by compounds that artificially induce the PIP cascade, or mimic it's products. A hypertonic shock, administered to intact B. vulgaris slices, further enhanced the phosphorylation of a 20 kD protein in the plasmalemma. Maximal IP{sub 3} content was found 10 min after hypertonic induction and maximal K{sup +} uptake was obtained 10 min later. The effect of a continuous hypertonic treatment on IP{sub 3} content, but not on K{sup +} uptake, was transient. Li{sup +} decreased the rate of IP{sub 3} metabolism.

• OSTI ID: 6643262
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHOSPHOLIPIDS
METABOLISM
PLANT TISSUES
BIOLOGICAL STRESS
BEETS
CPB
LITHIUM
PHOSPHORYLATION
POTASSIUM COMPOUNDS
SORGHUM
TRITIUM COMPOUNDS
ALKALI METAL COMPOUNDS
ALKALI METALS
BODY
CEREALS
CHEMICAL REACTIONS
ELEMENTS
ESTERS
FOOD
GRASS
HYDROGEN COMPOUNDS
LILIOPSIDA
LIPIDS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METALS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PLANTS
TISSUES
VEGETABLES
550201* - Biochemistry- Tracer Techniques
550501 - Metabolism- Tracer Techniques