Glyoxylate metabolism in the cyanobacterium Coccochloris peniocystis

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6642946

Norman, E G; Colman, B ^[1]

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1. York Univ., Toronto, Ontario (Canada)

The possible metabolism of glyoxylate via an incomplete glycolate pathway and a glyoxylate cycle in the cyanobacterium C. peniocystis was investigated. Levels of enzyme activities determined from partially purified preparations were: glycolate dehydrogenase, 1.18; isocitrate lyase, 6.67; malate synthetase, 1.03. Metabolism of {sup 14}C glycolate by intact cells was inhibited by the glycolate dehydrogenase inhibitor {alpha}-hydroxypyridyl methane sulfonate. Metabolism of {sup 14}C-glyoxylate was not inhibited by the amino-transferase inhibitor, aminooxyacetate, suggesting that formation of glycine is not an absolute requirement for glyoxylate metabolism. The lack of formation of labelled serine from {sup 14}C glyoxylate or {sup 14}C-glycine indicates that the glycolate pathway is incomplete. These results suggest that a glycolate pathway, such as found in higher plants, is absent in cyanobacteria and that some alternate pathway, possible a glyoxylate cycle, operates in the metabolism of glycolate pathway intermediates.

• OSTI ID: 6642946
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLYCOLIC ACID
METABOLISM
LIGASES
ENZYME ACTIVITY
LYASES
OXIDOREDUCTASES
CARBON 14 COMPOUNDS
ENZYME INHIBITORS
GLYCINE
TRACER TECHNIQUES
AMINO ACIDS
CARBOXYLIC ACIDS
ENZYMES
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
550501* - Metabolism- Tracer Techniques