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Title: Continuous production of ethanol by yeast ''immobilized'' in a membrane-contained fermentor

Abstract

A dialysate-feed, immobilized-cell dialysis continuous fermentation system was investigated as a method of relieving product inhibition in the conversion of glucose to ethanol by cells of Saccharomyces cerevisiae ATCC 4126. The substrate was fed into a continuous dialysate circuit and then into a batch fermentor circuit via diffusion through the microporous membranes of an intermediate dialyzer. Simultaneously, product was withdrawn from the fermentor circuit through the dialyzer membranes into the dialysate circuit and out in the effluent. Since the fermentor was operated without an effluent, the cells essentially were immobilized and converted substrate to product by maintenance metabolism. Contrary to prior results with this novel system for the continuous fermentation of lactose to lactate by lactobacillus cells, a steady state of yeast cells in the fermentor did not occur initially but was obtained by the depletion of medium nitrogen and the prevention of cell breakage, although the substrate and product concentrations then became unsteady. The inherent advantages of the system was offset in the ethanol fermentation by relatively low productivity, which appeared to be limited by membrane permeability. (Refs. 15).

Authors:
;
Publication Date:
Research Org.:
Dept of Microbiology and Public Health, Michigan State Univ, East Lansing, MI 48824
OSTI Identifier:
6630588
Resource Type:
Journal Article
Journal Name:
Biotechnol. Bioeng.; (United States)
Additional Journal Information:
Journal Volume: 26:3
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; ETHANOL; PRODUCTION; FERMENTATION; ENZYME INHIBITORS; CONTINUOUS CULTURE; DIALYSIS; IMMOBILIZED CELLS; MEMBRANES; PERMEABILITY; SACCHAROMYCES CEREVISIAE; ALCOHOLS; BIOCONVERSION; FUNGI; HYDROXY COMPOUNDS; MICROORGANISMS; ORGANIC COMPOUNDS; PLANTS; SACCHAROMYCES; SEPARATION PROCESSES; YEASTS; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)

Citation Formats

Kyung, K H, and Gerhardt, P. Continuous production of ethanol by yeast ''immobilized'' in a membrane-contained fermentor. United States: N. p., 1984. Web. doi:10.1002/bit.260260309.
Kyung, K H, & Gerhardt, P. Continuous production of ethanol by yeast ''immobilized'' in a membrane-contained fermentor. United States. https://doi.org/10.1002/bit.260260309
Kyung, K H, and Gerhardt, P. 1984. "Continuous production of ethanol by yeast ''immobilized'' in a membrane-contained fermentor". United States. https://doi.org/10.1002/bit.260260309.
@article{osti_6630588,
title = {Continuous production of ethanol by yeast ''immobilized'' in a membrane-contained fermentor},
author = {Kyung, K H and Gerhardt, P},
abstractNote = {A dialysate-feed, immobilized-cell dialysis continuous fermentation system was investigated as a method of relieving product inhibition in the conversion of glucose to ethanol by cells of Saccharomyces cerevisiae ATCC 4126. The substrate was fed into a continuous dialysate circuit and then into a batch fermentor circuit via diffusion through the microporous membranes of an intermediate dialyzer. Simultaneously, product was withdrawn from the fermentor circuit through the dialyzer membranes into the dialysate circuit and out in the effluent. Since the fermentor was operated without an effluent, the cells essentially were immobilized and converted substrate to product by maintenance metabolism. Contrary to prior results with this novel system for the continuous fermentation of lactose to lactate by lactobacillus cells, a steady state of yeast cells in the fermentor did not occur initially but was obtained by the depletion of medium nitrogen and the prevention of cell breakage, although the substrate and product concentrations then became unsteady. The inherent advantages of the system was offset in the ethanol fermentation by relatively low productivity, which appeared to be limited by membrane permeability. (Refs. 15).},
doi = {10.1002/bit.260260309},
url = {https://www.osti.gov/biblio/6630588}, journal = {Biotechnol. Bioeng.; (United States)},
number = ,
volume = 26:3,
place = {United States},
year = {Thu Mar 01 00:00:00 EST 1984},
month = {Thu Mar 01 00:00:00 EST 1984}
}