Picosecond spectroscopic studies of energy transfer in phycobiliproteins and model dye systems
Energy transfer was investigated in the ..cap alpha beta.. monomer and separated ..cap alpha.. and ..beta.. subunits of C-phycocyanin from Anabaena variabilis and Anacystis nidulans, using steady-state and picosecond spectroscopy. Fluorescence excitation polarization spectra were consistent with a sensitizing (s) - fluorescing (f) model using a Forster energy transfer mechanism. The rise in polarization across the absorption band towards longer wavelength for the ..beta.. subunit and the ..cap alpha beta.. monomer was attributed to energy transfer among the three chromophores in the ..cap alpha beta.. monomer and between the 2 chromophores in the ..beta.. subunit. The constant polarization of the ..cap alpha.. subunit, with one chromophore, is consistent with a lack of any possibility of energy transfer. Fluorescence emission maxima were at 640 nm for the ..cap alpha beta.. monomer and the separated subunits of Anabaena variabilis, and 645 nm for the ..beta.. subunit, 640 nm for the ..cap alpha.. subunit, and 644 nm for ..cap alpha beta.. monomer of Anacystis nidulans. We have shown that the labels s and f are not consistent with all the steady-state spectroscopic results. 171 refs., 32 figs., 15 tabs.
- Research Organization:
- California Univ., Berkeley (USA)
- DOE Contract Number:
- AC03-76SF00098
- OSTI ID:
- 6629064
- Report Number(s):
- LBL-22827; ON: DE87006933
- Resource Relation:
- Other Information: Thesis. Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALGAE
BIOLOGICAL EVOLUTION
PHOTOSYNTHETIC BACTERIA
PHYCOBILINS
CONFORMATIONAL CHANGES
FLUORESCENCE SPECTROSCOPY
MOLECULAR BIOLOGY
PHYCOBILIPROTEINS
ELECTRON TRANSFER
PHYCOBILISOMES
PHYCOCYANIN
GEL PERMEATION CHROMATOGRAPHY
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EMISSION SPECTROSCOPY
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PIGMENTS
PLANTS
PROTEINS
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SPECTROSCOPY
THYLAKOID MEMBRANE PROTEINS
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