Intracellular mediators of potassium-induced aldosterone secretion

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90102-W· OSTI ID:6623524

Ganguly, A; Chiou, S; Davis, J S ^[1]

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1. Univ. of South Florida, Tampa (USA)

We have investigated the intracellular messengers of potassium in eliciting aldosterone secretion in calf adrenal glomerulosa cells since there were unresolved issues relating to the role of phosphoinositides, cAMP and protein kinases. We observed no evidence of hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP{sub 2}) in {sup 3}H-inositol labeled alf adrenal cells or increase of cAMP in response to potassium. Addition of calcium channel blocker, nitrendipine after stimulating adrenal glomerulosa cells with potassium, markedly inhibited aldosterone secretion. A calmodulin inhibitor (W-7) produced greater reduction of aldosterone secretion than an inhibitor of protein kinase C (H-7). These results suggest that a rise in cytosolic free calcium concentration through voltage-dependent calcium channel and calmodulin are the critical determinants of aldosterone secretion stimulated by potassium.

• OSTI ID: 6623524
• Journal Information: Life Sciences; (USA), Vol. 46:3, Issue 3; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALDOSTERONE
SECRETION
PORINS
BIOCHEMICAL REACTION KINETICS
ADRENAL GLANDS
CALMODULIN
CALVES
ENZYME INHIBITORS
INHIBITION
POTASSIUM
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
ALDEHYDES
ALKALI METALS
ANIMALS
BODY
CATTLE
CORTICOSTEROIDS
DOMESTIC ANIMALS
ELEMENTS
ENDOCRINE GLANDS
GLANDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
KINETICS
MAMMALS
MEMBRANE PROTEINS
METALS
MINERALOCORTICOIDS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROTEINS
REACTION KINETICS
RUMINANTS
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques