Control of nitrogenase recovery from oxygen inactivation by ammonia in the cyanobacterium anabaena sp. strain CA (ATCC 33047)

Journal Article · Tue May 01 00:00:00 EDT 1990 · Journal of Bacteriology; (USA)

OSTI ID:6622233

Smith, R L; Van Baalen, C ^[1]; Tabita, F R ^[2]

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1. Univ. of Texas Marine Science Institute, Port Aransas (USA)
2. Univ. of Texas, Austin (USA) Ohio State Univ., Columbus (USA)

The control of nitrogenase recovery from inactivation by oxygen was studied in Anabaena sp. strain CA (ATCC 33047). Nitrogenase activity (acetylene reduction) in cultures grown in 1% CO{sub 2} in air was inhibited by exposure to 1% CO{sub 2}-99% O{sub 2} and allowed to recover in the presence of high oxygen tensions. Cultures exposed to hyperbaric levels of oxygen in the presence of 10 mM NH{sub 4}NO{sub 3} were incapable of regaining nitrogenase activity, whereas control cultures returned to 65 to 80% of their original activity within about 3 h after exposure to high oxygen tension. In contrast to the regulation of heterocyst differentiation and nitrogenase synthesis, recovery from oxygen inactivation in this organism was shown to be under the control of NH{sub 4}{sup +} rather than NO{sub 3}{sup {minus}}.

• OSTI ID: 6622233
• Journal Information: Journal of Bacteriology; (USA), Vol. 172:5; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMMONIA
BIOLOGICAL EFFECTS
CYANOBACTERIA
ENZYME ACTIVITY
OXYGEN
TOXICITY
CARBON DIOXIDE
INACTIVATION
NITROGENASE
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
ELEMENTS
ENZYMES
HYDRIDES
HYDROGEN COMPOUNDS
MICROORGANISMS
NITRO-GROUP DEHYDROGENASES
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NONMETALS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
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