Uptake of thymidine labeled on carbon 2: A potential index of liver regeneration by positron emission tomography

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · Hepatology (Baltimore); (USA)

DOI:https://doi.org/10.1002/hep.1840120118· OSTI ID:6620168

Vander Borght, T M; Lambotte, L E; Pauwels, S A; Dive, C C ^[1]

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1. Univ. of Louvain, Brussels (Belgium)

Noninvasive measurement of liver regeneration with positron emission tomography has been attempted with 11C-thymidine; however, results were unsatisfactory using thymidine labeled on its methyl group. To evaluate whether the specificity of the method could be improved by modifying the labeling position of the tracer, thymidine labeled on its methyl group with 3H and thymidine labeled on its carbon 2 with 14C were injected in 22 hepatectomized rats either 1 hr (when DNA synthesis is not increased) or 24 hr after the surgical procedure (when the rate of DNA synthesis is maximal). Liver samples taken 10, 30 and 120 min after injection showed that, in contrast to 3H-radioactivity, 14C-radioactivity measured in whole tissue allowed a clear discrimination between regenerating and nonregenerating livers. In addition, 14C-radioactivity measured in whole tissue of regenerating livers correlated with the DNA radioactivity 10, 30 and 120 min after injection of the tracer. In contrast, no such correlation was found with the methyl-labeled thymidine. Analysis of the radioactive material present in the non-DNA fraction using ion exchange disks and high-performance liquid chromatography showed that 2-C-labeled thymidine was incorporated into DNA without accumulation of labeled metabolites whereas, for the methyl-labeled thymidine, almost all radioactivity was related to degradative products. Therefore the evaluation of the liver regeneration with the 2-C-labeled thymidine, which does not require cellular fractionation, should be suited for noninvasive measurement with positron emission tomography.

• OSTI ID: 6620168
• Journal Information: Hepatology (Baltimore); (USA), Vol. 12:1; ISSN 0270-9139
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
LIVER
BIOLOGICAL REGENERATION
THYMIDINE
UPTAKE
CARBON 11
CARBON 14 COMPOUNDS
DNA REPLICATION
ION EXCHANGE
LIQUID COLUMN CHROMATOGRAPHY
POSITRON COMPUTED TOMOGRAPHY
RATS
REGRESSION ANALYSIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BODY
CARBON ISOTOPES
CHROMATOGRAPHY
COMPUTERIZED TOMOGRAPHY
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
EMISSION COMPUTED TOMOGRAPHY
EVEN-ODD NUCLEI
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MATHEMATICS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIOISOTOPES
RECOVERY
RIBOSIDES
RODENTS
SEPARATION PROCESSES
STATISTICS
TOMOGRAPHY
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics