Interaction of metal ions with cadmium-induced cellular toxicity
Interactions between Cd and other metal ions are important from both nutritional and toxicological aspects. As Cd is toxic to isolated hepatocytes, these cells can be used to investigate the effects of other metals on Cd-induced cellular injury. Isolated hepatocytes were incubated at 37/sup 0/C with vehicle (saline); Cd (200 or 400 ..mu..M); or Cd plus Cr, Mn, Zn, Ni, Pb, Se, or Fe (200 to 1000 ..mu..M). Evidence of cellular injury was assessed by loss of intracellular K/sup +/ and aspartate aminotransferase from the hepatocytes. Effects on lipid peroxidation, as measured by concentration of thiobarbituric acid reactants, were assessed. Uptake of /sup 109/Cd and interaction of the other metal ions with this accumulation were also quantitated. Cell injury due to Cd was consistently reduced by Cr, Mn, Zn, Pb, and Fe. Lipid peroxidation due to Cd was inhibited by Cr, Mn, and Zn. All the metals except Ni produced an increase in the amount of Cd accumulated by hepatocytes. There was no consistent relation between reduction of cellular toxicity and either inhibition of lipid peroxidation or uptake of Cd. These experiments show that (1) protective properties of some metals seen in vivo can be demonstrated at the cellular level and (2) protective effects of metals in general on Cd-induced cellular toxicity are not due to a decrease in either Cd uptake or lipid peroxidation.
- Research Organization:
- Univ. of Kansas, Kansas City
- OSTI ID:
- 6619216
- Journal Information:
- J. Toxicol. Environ. Health; (United States), Vol. 7:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINOTRANSFERASES
BIOCHEMICAL REACTION KINETICS
CADMIUM
TOXICITY
CHROMIUM
BIOLOGICAL EFFECTS
IRON
LEAD
LIVER
SENSITIVITY
MANGANESE
NICKEL
ZINC
CADMIUM 109
INTRACELLULAR DIGESTION
LIPIDS
METABOLISM
POTASSIUM
UPTAKE
ALKALI METALS
BETA DECAY RADIOISOTOPES
BODY
CADMIUM ISOTOPES
DIGESTION
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
ENZYMES
EVEN-ODD NUCLEI
GLANDS
INTERMEDIATE MASS NUCLEI
ISOTOPES
KINETICS
METALS
NITROGEN TRANSFERASES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
REACTION KINETICS
TRANSFERASES
TRANSITION ELEMENTS
YEARS LIVING RADIOISOTOPES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550200 - Biochemistry
550300 - Cytology