Phytochrome in photosynthetically competent plants characterization by monoclonal antibodies: Progress report

Technical Report · Sun Mar 01 00:00:00 EST 1987

OSTI ID:6618888

Pratt, L H

It is evident that phytochrome in green oats differs more markedly from that in etiolated oats than could have been anticipated. More effort has gone into characterization of green-oat phytochrome, including elimination of likely artifacts that might have been responsible for the observed differences with etiolated-oat phytochrome, and into the generation of new probes for green-oat phytochrome. The liability of green-oat phytochrome has increased the difficulty of this work. Immunochemical differences between etiolated- and green-oat phytochrome have been elucidated. A domain on green-oat phytochrome that undergoes a change upon phototransformation of at least etiolated-oat phytochrome has been discovered. A lambda gt11 cDNA library has been prepared from size-enriched poly(A)/sup +/-mRNA isolated from green oat shoots and has been screened immunochemically. An amplified ELISA protocol suitable for screening hybridoma supernatants against crude green-oat phytochrome preparations has been developed. Monoclonal antibodies directed to green-oat phytochrome have been identified. 5 refs.

• Research Organization: Georgia Univ., Athens (USA). Dept. of Botany
• DOE Contract Number: AS09-81ER10925
• OSTI ID: 6618888
• Report Number(s): DOE/ER/10925-5; ON: DE87009342
• Resource Relation: Other Information: Portions of this document are illegible in microfiche products
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
59 BASIC BIOLOGICAL SCIENCES
PHYTOCHROMES
FRACTIONATION
ENZYME IMMUNOASSAY
LIQUID COLUMN CHROMATOGRAPHY
MONOCLONAL ANTIBODIES
OATS
ANTIBODIES
CEREALS
CHROMATOGRAPHY
GRASS
IMMUNOASSAY
ORGANIC COMPOUNDS
PIGMENTS
PLANTS
PROTEINS
SEPARATION PROCESSES
140505* - Solar Energy Conversion- Photochemical
Photobiological
& Thermochemical Conversion- (1980-)
550200 - Biochemistry