ATP-sensitive K/sup +/ channels that are blocked by hypoglycemia-inducing sulfonylureas in insulin-secreting cells are activated by galanin, a hyperglycemia-inducing hormone

Journal Article · Mon Feb 01 00:00:00 EST 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.4.1312· OSTI ID:6618881

de Weille, J; Schmid-Antomarchi, H; Fosset, M; Lazdunski, M

The action of the hyperglycemia-inducing hormone galanin, a 29-amino acid peptide names from its N-terminal glycine and C-terminal amidated alanine, was studied in rat insulinoma (RINm5F) cells using electrophysiological and /sup 86/Rb/sup +/ flux techniques. Galanin hyperpolarizes and reduces spontaneous electrical activity by activating a population of APT-sensitive K/sup +/ channels with a single-channel conductance of 30 pS (at -60 mV). Galanin-induced hyperpolarization and reduction of spike activity are reversed by the hypoglycemia-inducing sulfonylurea glibenclamine. Glibenclamide blocks the galanin-activated ATP-sensitive K/sup +/ channel. /sup 86/Rb/sup +/ efflux from insulinoma cells is stimulated by galanin in a dose-dependent manner. The half-maximum value of activation is found at 1.6 nM. Galanin-induced /sup 86/Rb/sup +/ efflux is abolished by glibenclamide. The half-maximum value of inhibition is found at 0.3 nM, which is close to the half-maximum value of inhibition of the ATP-dependent K/sup +/ channel reported earlier. /sup 86/Rb/sup +/ efflux studies confirm the electrophysiological demonstration that galanin activates and ATP-dependent K/sup +/ channel.

• Research Organization: Centre National de la Recherche Scientifique, Nice (France)
• OSTI ID: 6618881
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PEPTIDE HORMONES
BIOLOGICAL EFFECTS
RUBIDIUM 86
MEMBRANE TRANSPORT
TUMOR CELLS
BIOELECTRICITY
ALANINES
ATP
GLYCINE
HYPERGLYCEMIA
INSULIN
POLARIZATION
RATS
UREA
ALKALI METAL ISOTOPES
AMIDES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
ELECTRICITY
HORMONES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIOISOTOPES
RODENTS
RUBIDIUM ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques