Sources of propionate for the biogenesis of ethyl-braced insect juvenile hormones: role of isoleucine and valine
Corpora allata from adult female Manduca sexta biosynthesis the sesquiterpenoid juvenile hormone (JH) III and the unusual ethyl-branched homologue JH II in vitro. The authors maintained corpora allata in medium 199 using (methyl-/sup 3/H)methionine as the source of the JH methyl ester moiety and as a mass marker. This allowed measurement of the relative contributions of /sup 14/C-labeled precursors to the biogenesis of JH II and III carbon skeletons. They showed efficient incorporation of a propionate equivalent, from isoleucine or valine catabolism, into the ethyl-branched portion of JH II, using double-label liquid scintillation counting of isolated JHs and gas chromatography/mass spectrometry with selected ion monitoring of JH deuteromethoxyhydrin derivatives. Methionine was a poor source of propionate for JH II biosynthesis, while glucose, succinate, threonine, and ..beta..-alanine did not contribute propionate at all. Leucine, isoleucine, and glucose incorporated into JH III and the acetate-derivative portion of JH II.
- Research Organization:
- Zoecon Research Institute, Palo Alto, CA (USA)
- OSTI ID:
- 6618810
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:22
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HORMONES
BIOSYNTHESIS
PROPIONIC ACID
BIOLOGICAL PATHWAYS
TRITIUM COMPOUNDS
ALANINES
CARBON 14 COMPOUNDS
GAS CHROMATOGRAPHY
GLUCOSE
INSECTS
JUVENILES
LEUCINE
MASS SPECTROSCOPY
METHIONINE
THREONINE
VALINE
ALDEHYDES
AMINO ACIDS
ANIMALS
ARTHROPODS
CARBOHYDRATES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DRUGS
HEXOSES
HYDROXY ACIDS
INVERTEBRATES
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
SACCHARIDES
SEPARATION PROCESSES
SPECTROSCOPY
SYNTHESIS
550201* - Biochemistry- Tracer Techniques