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Title: A novel zinc finger gene on human chromosome 1qter that is alternatively spliced in human tissues and cell lines

Abstract

DNA-binding proteins that share the conserved C[sub 2]-H[sub 2] zinc finger motif have been shown to have important roles as transcriptional regulators of gene expression and have been implicated in several hereditary human diseases. In order to define potential candidate genes for inherited disorders characterized by aberrant gene expression, the authors utilized Kruppel-related sequences to isolate zinc finger-containing cDNAs. They isolated and characterized two novel zinc finger-encoding cDNAs from a human hepatoblastoma cell line, which demonstrate DNA sequence homology to a recently described human Kruppel-related gene HZF-3 and appear to be derived from a single gene by alternate mRNA splicing. This gene, denoted HZF-16,' gives rise to at least two gene products. One cDNA (i.e., HZF-16.2) has nine zinc finger domains, while alternative splicing of the message gives rise to a smaller product (i.e., HZF-16.1) that has four domains. Despite the internal splicing event, both the 5[prime]- and 3[prime]-untranslated sequences in both cDNAs are identical, as are the first three domains. In the HZF-16.1 cDNA, the fourth zinc finger domain is a fusion product of domains four and nine of HZF-16.2 and could potentially give rise to a new DNA-binding specificity. These alternatively spliced transcripts are differentially regulated in humanmore » tissues and transformed cell lines and show a different distribution of expression between human cell lines and normal human tissue. This novel gene was mapped to human chromosome 1q44 by chromosomal in situ suppression hybridization and thus represents a candidate gene for trisomy 1q syndrome and for several other disorders. 40 refs., 6 figs.« less

Authors:
; ;  [1]
  1. Salk Inst. for Biological Studies, La Jolla, CA (United States)
Publication Date:
OSTI Identifier:
6615966
Resource Type:
Journal Article
Journal Name:
American Journal of Human Genetics; (United States)
Additional Journal Information:
Journal Volume: 52:1; Journal ID: ISSN 0002-9297
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENE REGULATION; GENES; HUMAN CHROMOSOMES; MAPPING; PROTEINS; DISEASES; DNA; DNA HYBRIDIZATION; DNA SEQUENCING; ZINC; CHROMOSOMES; ELEMENTS; HYBRIDIZATION; METALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics

Citation Formats

Saleh, M, Selleri, L, and Evans, G A. A novel zinc finger gene on human chromosome 1qter that is alternatively spliced in human tissues and cell lines. United States: N. p., 1993. Web.
Saleh, M, Selleri, L, & Evans, G A. A novel zinc finger gene on human chromosome 1qter that is alternatively spliced in human tissues and cell lines. United States.
Saleh, M, Selleri, L, and Evans, G A. 1993. "A novel zinc finger gene on human chromosome 1qter that is alternatively spliced in human tissues and cell lines". United States.
@article{osti_6615966,
title = {A novel zinc finger gene on human chromosome 1qter that is alternatively spliced in human tissues and cell lines},
author = {Saleh, M and Selleri, L and Evans, G A},
abstractNote = {DNA-binding proteins that share the conserved C[sub 2]-H[sub 2] zinc finger motif have been shown to have important roles as transcriptional regulators of gene expression and have been implicated in several hereditary human diseases. In order to define potential candidate genes for inherited disorders characterized by aberrant gene expression, the authors utilized Kruppel-related sequences to isolate zinc finger-containing cDNAs. They isolated and characterized two novel zinc finger-encoding cDNAs from a human hepatoblastoma cell line, which demonstrate DNA sequence homology to a recently described human Kruppel-related gene HZF-3 and appear to be derived from a single gene by alternate mRNA splicing. This gene, denoted HZF-16,' gives rise to at least two gene products. One cDNA (i.e., HZF-16.2) has nine zinc finger domains, while alternative splicing of the message gives rise to a smaller product (i.e., HZF-16.1) that has four domains. Despite the internal splicing event, both the 5[prime]- and 3[prime]-untranslated sequences in both cDNAs are identical, as are the first three domains. In the HZF-16.1 cDNA, the fourth zinc finger domain is a fusion product of domains four and nine of HZF-16.2 and could potentially give rise to a new DNA-binding specificity. These alternatively spliced transcripts are differentially regulated in human tissues and transformed cell lines and show a different distribution of expression between human cell lines and normal human tissue. This novel gene was mapped to human chromosome 1q44 by chromosomal in situ suppression hybridization and thus represents a candidate gene for trisomy 1q syndrome and for several other disorders. 40 refs., 6 figs.},
doi = {},
url = {https://www.osti.gov/biblio/6615966}, journal = {American Journal of Human Genetics; (United States)},
issn = {0002-9297},
number = ,
volume = 52:1,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1993},
month = {Fri Jan 01 00:00:00 EST 1993}
}