Hepatic glutathione and glutathione S-transferase in selenium deficiency and toxicity in the chick

Miscellaneous · Sun Jan 01 00:00:00 EST 1989

OSTI ID:6612577

Kim, Y S

First, the hepatic activity of GSH-T{sub CDNB} was increased only under conditions of severe oxidative stress produced by combined Se- and vitamin E (VE)-deficiency, indicating that VE also affects GSH metabolism. Second, the incorporation of {sup 35}S-methionine into GSH and protein was about 4- and 2-fold higher, respectively, in Se- and VE-deficient chick hepatocytes as compared to controls. Third, chicks injected with the glutathione peroxidase (SeGSHpx) inhibitor, aurothioglucose (AuTG), showed increase hepatic GSH-T{sub CDNB} activity and plasma GSH concentration regardless of their Se status. Fourth, the effect of ascorbic acid (AA), on GSH metabolism was studied. Chicks fed 1000 ppm AA showed decreased hepatic GSH concentration compared to chicks fed no AA in a Se- and VE-deficient diet. Fifth, chicks fed excess Se showed increase hepatic activity of GSH-T{sub CDNB} and GSH concentration regardless of VE status.

• Research Organization: Cornell Univ., Ithaca, NY (USA)
• OSTI ID: 6612577
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
GLUTATHIONE
METABOLISM
SELENIUM
TOXICITY
TRANSFERASES
ENZYME ACTIVITY
CHICKENS
ENZYME INHIBITORS
LIVER CELLS
METHIONINE
NUTRITIONAL DEFICIENCY
SULFUR 35
TRACER TECHNIQUES
VITAMIN E
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIRDS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
ENZYMES
EVEN-ODD NUCLEI
FOWL
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
SEMIMETALS
SOMATIC CELLS
SULFUR ISOTOPES
VERTEBRATES
VITAMINS
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