Protein synthesis inhibitor from potato tuber

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6610858

Romaen, R ^[1]

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1. Ciudad Universitaria (Mexico)

A protein fraction capable of inhibit in vitro protein synthesis was found in potato tubers in fresh and wounded tissue. Inhibitor activity from fresh tissue decays with wounding. Inhibition activity was detected absorbed to ribsomal fraction and cytosol of potato tuber tissue by a partially reconstituted in vitro system from potato tuber and wheat germ. Adsorbed ribosomal fraction was more suitable of purification. This fraction was washed from ribosomes with 0.3M KCl, concentrated with ammonium sulfate precipitation and purified through sephadex G100 and sephadex G-75 columns chromatography. After 61 fold purification adsorbed protein fraction can inhibit germination of maize, wheat and sesame seeds, as well as {sup 3}H-leucine incorporation into protein by imbibed maize embryos. Inhibition activity was lost by temperature, alkali and protease-K hydrolysis. Preliminar analysis could not show presence of reductor sugars. Physiological role of this inhibitor in relation to rest and active tissue remains to be studied.

• OSTI ID: 6610858
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
BIOSYNTHESIS
SEEDS
GERMINATION
FRACTIONATION
INHIBITION
INJURIES
LEUCINE
RIBOSOMES
SOLANUM TUBEROSUM
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
PLANTS
SEPARATION PROCESSES
SOLANUM
SYNTHESIS
550201* - Biochemistry- Tracer Techniques