Regulation of protein synthesis during photomorphogenesis of gametophytes of the fern Onoclea sensibilis
- Ohio State Univ., Columbus (USA)
Gametophytes of the fern Onoclea sensibilis grow as filaments in the dark and in red light and become planar in blue light. Pulse-labeling 4-day-old gametophytes with ({sup 35}S)methionine at different times after transfer to dark, red, and blue light environments revealed higher rates of amino acid uptake and protein synthesis in blue light than in red light or in the dark. Characterization of the extant and newly synthesized soluble proteins by one- and two-dimensional gel electrophoresis showed that the patterns of protein accumulation and synthesis in gametophytes exposed to short periods of red or blue light were qualitatively indistinguishable from those of gametophytes maintained in the dark. However, some striking increases and decreases in the levels of certain polypeptides were noted and these changes were accentuated during continued growth of gametophytes in the different environments. The results show that photomorphogenesis of gametophytes of O. sensibilis is associated with quantitative rather than qualitative changes in the population of mRNAs available for translation.
- OSTI ID:
- 6610650
- Journal Information:
- Plant Physiology; (USA), Vol. 90:4; ISSN 0032-0889
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FERNS
PHOTOSYNTHESIS
PROTEINS
BIOSYNTHESIS
METHIONINE
PLANT GROWTH
SULFUR 35
TRACER TECHNIQUES
TWO-DIMENSIONAL ELECTROPHORESIS
VISIBLE RADIATION
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
EVEN-ODD NUCLEI
GROWTH
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOTOCHEMICAL REACTIONS
PLANTS
RADIATIONS
RADIOISOTOPES
SULFUR ISOTOPES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques