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Title: Oxidation of indole-3-acetic acid to oxindole-3-acetic acid by etiolated and green corn tissues

Abstract

Etiolated corn tissues oxidase indole-3-acetic acid (IAA) to oxindole-3-acetic acid (OxIAA). This oxidation results in loss of auxin activity and may plant a role in regulating IAA-stimulated growth. The enzyme has been partially purified and characterized and shown to require O{sub 2}, and a heat-stable lipid-soluble corn factor which can be replaced by linolenic or linoleic acids in the oxidation of IAA. Corn oil was tested as a cofactor in the IAA oxidation reaction. Corn oil stimulated enzyme activity by 30% while trilinolein was inactive. The capacity of green tissue to oxidize IAA was examined by incubating leaf sections from 2 week old light-grown corn seedlings with {sup 14}C-IAA. OxIAA and IAA were separated from other IAA metabolites on a 3 ml anion exchange column. Of the IAA taken up by the sections, 13% was oxidized to OxIAA. This is the first evidence that green tissue of corn may also regulate IAA levels by oxidizing IAA to OxIAA.

Authors:
 [1]
  1. Michigan State Univ., East Lansing (USA)
Publication Date:
OSTI Identifier:
6610066
Resource Type:
Journal Article
Journal Name:
Plant Physiology, Supplement; (USA)
Additional Journal Information:
Journal Volume: 89:4; Journal ID: ISSN 0079-2241
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AUXINS; METABOLISM; MAIZE; PLANT GROWTH; CARBON 14 COMPOUNDS; CHEMICAL COMPOSITION; FRACTIONATION; ION EXCHANGE CHROMATOGRAPHY; PLANT TISSUES; TRACER TECHNIQUES; BODY; CEREALS; CHROMATOGRAPHY; GRASS; GROWTH; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LILIOPSIDA; MAGNOLIOPHYTA; PLANT GROWTH REGULATORS; PLANTS; SEPARATION PROCESSES; TISSUES; 550501* - Metabolism- Tracer Techniques

Citation Formats

Reinecke, D. Oxidation of indole-3-acetic acid to oxindole-3-acetic acid by etiolated and green corn tissues. United States: N. p., 1989. Web.
Reinecke, D. Oxidation of indole-3-acetic acid to oxindole-3-acetic acid by etiolated and green corn tissues. United States.
Reinecke, D. 1989. "Oxidation of indole-3-acetic acid to oxindole-3-acetic acid by etiolated and green corn tissues". United States.
@article{osti_6610066,
title = {Oxidation of indole-3-acetic acid to oxindole-3-acetic acid by etiolated and green corn tissues},
author = {Reinecke, D},
abstractNote = {Etiolated corn tissues oxidase indole-3-acetic acid (IAA) to oxindole-3-acetic acid (OxIAA). This oxidation results in loss of auxin activity and may plant a role in regulating IAA-stimulated growth. The enzyme has been partially purified and characterized and shown to require O{sub 2}, and a heat-stable lipid-soluble corn factor which can be replaced by linolenic or linoleic acids in the oxidation of IAA. Corn oil was tested as a cofactor in the IAA oxidation reaction. Corn oil stimulated enzyme activity by 30% while trilinolein was inactive. The capacity of green tissue to oxidize IAA was examined by incubating leaf sections from 2 week old light-grown corn seedlings with {sup 14}C-IAA. OxIAA and IAA were separated from other IAA metabolites on a 3 ml anion exchange column. Of the IAA taken up by the sections, 13% was oxidized to OxIAA. This is the first evidence that green tissue of corn may also regulate IAA levels by oxidizing IAA to OxIAA.},
doi = {},
url = {https://www.osti.gov/biblio/6610066}, journal = {Plant Physiology, Supplement; (USA)},
issn = {0079-2241},
number = ,
volume = 89:4,
place = {United States},
year = {Sat Apr 01 00:00:00 EST 1989},
month = {Sat Apr 01 00:00:00 EST 1989}
}