Detection of pentachlorophenol and its glucuronide and sulfate conjugates in fish bile and exposure water
The glucuronide and sulfate conjugates of pentachlorophenol (PCP) that were present in the bile and exposure water of goldfish (Carassius auratus) were used to develop methodology to quantify PCP and its metabolites. Reverse phase HPLC with radioactivity detection separated PCP and its metabolites, and was used to verify a method of quantification that used differential extraction and scintillation counting. Extractions of aqueous phase at pH 2 or 8, with butanol, ethyl acetate, or ether indicated that ether at pH 8 best separated PCP from its metabolites. The sulfate conjugate of PCP was the major metabolite produced when goldfish were exposed to 125 micrograms UC-PCP/l. It was present primarily in the exposure water, but also appeared in the bile.
- Research Organization:
- State Univ., Pullman, WA (USA)
- OSTI ID:
- 6608147
- Journal Information:
- J. Environ. Sci. Health, Part B; (United States), Vol. 23:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CHLORINATED AROMATIC HYDROCARBONS
METABOLISM
GOLDFISH
CONTAMINATION
BILE
CARBON 14 COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
METABOLITES
SCINTILLATION COUNTING
SOLVENT EXTRACTION
SULFATES
TRACER TECHNIQUES
WATER POLLUTION
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BIOLOGICAL MATERIALS
BODY FLUIDS
CHROMATOGRAPHY
COUNTING TECHNIQUES
EXTRACTION
FISHES
HALOGENATED AROMATIC HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MATERIALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
POLLUTION
SEPARATION PROCESSES
SULFUR COMPOUNDS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques