Gas-chromatographic assay for heme oxygenase activity

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6603502

Sunderman, Jr, F W; Downs, J R; Reid, M C; Bibeau, L M

The authors have developed an improved assay for microsomal heme oxygenase activity, based on the enzymic release of CO from the ..cap alpha..-methene bridge of hemin and the quantitation of CO by gas chromatography. The detection limit for heme oxygenase activity was approximately 1 nmol/h per milligram of microsomal protein. Gas-chromatographic assays of heme oxygenase activities in rat tissues correlated well (r = 0.94) with results by a spectrophotometric assay based on bilirubin production. Heme oxygenase activity was increased 10-fold in kidney microsomes and threefold in liver microsomes from rats killed 17 h after subcutaneous injection of NiCl/sub 2/ (0.5 mmol/kg body wt.). These findings illustrate the efficacy of the gas-chromatographic assay for measuring xenobiotic effects on heme oxygenase activity.

• Research Organization: Univ. of Connecticut School of Medicine, Farmington
• OSTI ID: 6603502
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 28:10
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBON MONOXIDE
GAS CHROMATOGRAPHY
NICKEL CHLORIDES
BIOLOGICAL EFFECTS
OXYGENASES
ENZYME ACTIVITY
HEME
KIDNEYS
LIVER
MICROSOMES
RATS
ANIMALS
BODY
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHALCOGENIDES
CHLORIDES
CHLORINE COMPOUNDS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAMMALS
NICKEL COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PIGMENTS
PORPHYRINS
RODENTS
SEPARATION PROCESSES
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
550200* - Biochemistry
560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)