Further studies on the intermediary metabolism of bone in vitro and a monoclonal cell line (MMB-1) derived from bone: effects of parathyroid hormone and acetazolamide
The mechanism/mechanisms by which PTH affects Ca homeostasis between blood and bone have not been clearly established. Most studies of metabolic acid production in bone took place during the late 1950s and early 1960s. Since that time, assay techniques for metabolic acid production have been improved for greater sensitivity, more has been learned as to how PTH mediates its cellular responses, and techniques for cell isolation and culture have dramatically improved. These improvements have made possible new approaches to the study of short term effects of PTH on metabolic acid production in bone. Chapter 1 explores the potential of bone to utilize substrates other than glucose for metabolic energy transfer. Chapter 2 characterizes glucose metabolism in mouse calvaria in vitro and explores effects of PTH, acetazolamide, and C1 13,850 on calvaria oxidative metabolism. Chapter 3 describes PTH effects on glucose metabolism of MMB-1 cells, a monoclonal cell line reported to possess osteoblast-like characteristics.
- Research Organization:
- Rochester Univ., NY (USA)
- DOE Contract Number:
- AC02-76EV03490
- OSTI ID:
- 6603172
- Report Number(s):
- DOE/EV/03490-2432; ON: DE84016249
- Resource Relation:
- Other Information: Portions are illegible in microfiche products. Thesis
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMIDES
BIOLOGICAL EFFECTS
BONE CELLS
CELL CULTURES
BONE TISSUES
METABOLISM
PARATHORMONE
CALCIUM
CARBON 14 COMPOUNDS
GLUCOSE
TRACER TECHNIQUES
ALDEHYDES
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMAL TISSUES
BODY
CARBOHYDRATES
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
ELEMENTS
HEXOSES
HORMONES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HORMONES
SACCHARIDES
SOMATIC CELLS
TISSUES
550501* - Metabolism- Tracer Techniques