Rapid susceptibility testing of Mycobacterium tuberculosis by bioluminescence assay of mycobacterial ATP

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · Antimicrob. Agents Chemother.; (United States)

DOI:https://doi.org/10.1128/AAC.32.8.1208· OSTI ID:6602940

Nilsson, L E; Hoffner, S E; Ansehn, S

Mycobacterial growth was monitored by bioluminescence assay of mycobacterial ATP. Cultures of Mycobacterium tuberculosis H37Rv and of 25 clinical isolates of the same species were exposed to serial dilutions of ethambutol, isoniazid, rifampin, and streptomycin. A suppression of ATP, indicating growth inhibition, occurred for susceptible but not resistant strains within 5 to 7 days of incubation. Breakpoint concentrations between susceptibility and resistance were determined by comparing these results with those obtained by reference techniques. Full agreement was found in 99% of the assays with the resistance ratio method on Lowenstein-Jensen medium, and 98% of the assays were in full agreement with the radiometric system (BACTEC). A main advantage of the bioluminescence method is its rapidity, with results available as fast as with the radiometric system but at a lower cost and without the need for radioactive culture medium. The method provides kinetic data concerning drug effects within available in vivo drug concentrations and has great potential for both rapid routine susceptibility testing and research applications in studies of drug effects on mycobacteria.

• Research Organization: University Hospital, Linkoeping (Sweden)
• OSTI ID: 6602940
• Journal Information: Antimicrob. Agents Chemother.; (United States), Vol. 32:8
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ATP
BIOASSAY
MYCOBACTERIUM TUBERCULOSIS
GROWTH
TUBERCULOSIS
PATHOGENESIS
BIOLUMINESCENCE
DOSE-RESPONSE RELATIONSHIPS
INFECTIVITY
ISONIAZID
RADIOMETRIC ANALYSIS
STREPTOMYCIN
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMICROBIAL AGENTS
BACTERIA
BACTERIAL DISEASES
CHEMICAL ANALYSIS
DISEASES
DRUGS
HYDRAZIDES
INFECTIOUS DISEASES
LUMINESCENCE
MICROORGANISMS
MYCOBACTERIUM
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
550901* - Pathology- Tracer Techniques