Intimal permeability evaluated in a short-term organ culture of diabetic guinea pig aorta
A novel short-term organ culture system was used to evaluate intimal permeability changes by measuring aortic (/sup 14/C)methylated albumin accumulation. Aortic plugs were removed from the upper thoracic aorta of male guinea pigs and maintained in serum-free media. The accumulation of (/sup 14/C)albumin in the intimal-medial layer was determined after a 5 h incubation. In preliminary studies, albumin recovered from intimal-injured aortic plugs was significantly greater than those from non-injured plugs. Aortic plugs from streptozotocin-treated guinea pigs, diabetic for 3 weeks, also accumulated significantly more (/sup 14/C)albumin than plugs from nondiabetic controls. Histological changes were not observed in the aorta of either the diabetic or control group. A strong significant inverse correlation was found between plasma ascorbic acid levels and (/sup 14/C)-activity recovered from aortic plugs. This study demonstrates a simple and rapid method for assessing aortic permeability changes under a well-defined in vitro system, and suggests that vascular permeability changes in the streptozotocin-diabetic guinea pig may be associated with an ascorbic acid deficit.
- Research Organization:
- Univ. of Mississippi, University (USA)
- OSTI ID:
- 6602926
- Journal Information:
- Artery (Fulton, Mich.); (United States), Vol. 15:6
- Country of Publication:
- United States
- Language:
- English
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ALBUMINS
BIOLOGICAL ACCUMULATION
AORTA
PERMEABILITY
DIABETES MELLITUS
PATHOGENESIS
CARBON 14 COMPOUNDS
GUINEA PIGS
IN VITRO
TRACER TECHNIQUES
ANIMALS
ARTERIES
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
DISEASES
ENDOCRINE DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METABOLIC DISEASES
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
VERTEBRATES
550901* - Pathology- Tracer Techniques