Mechanism of action of the copper(I) complex of 2,9-dimethyl-1,10-phenanthroline on Mycoplasma gallisepticum
Evidence was found that the inhibitory action of Cu(DMP)/sub 2/NO/sub 3/, the copper(I) complex of 2,9-dimethyl-1,10-phenanthroline (DMP), on Mycoplasma gallisepticum is a consequence of the ultimate toxicity of copper, and not that of the ligand, DMP. From uptake studies with radiolabeled /sup 67/Cu and (/sup 14/C)DMP, we concluded that significantly more copper than DMP is bound to the mycoplasmal cell. It appeared that dissociation of Cu(DMP)2+ occurred shortly after interaction with the cell membrane. Copper was transported across the cytoplasmic membrane. A strong dependence of copper uptake on the incubation medium was observed in the absence of DMP. The main function of the ligand DMP appeared to be as a vehicle for the transport of copper from nontoxic copper-medium complexes to membrane-buried cellular ligands.
- Research Organization:
- Departments of Medicinal Chemistry, Universiteit, Amsterdam, The Netherlands
- OSTI ID:
- 6601774
- Journal Information:
- Antimicrob. Agents Chemother.; (United States), Vol. 21:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
COPPER 67
COPPER COMPLEXES
BIOLOGICAL EFFECTS
MYCOPLASMA
SENSITIVITY
ANTIMICROBIAL AGENTS
BIOLOGICAL PATHWAYS
LIGANDS
MEMBRANE TRANSPORT
TOXICITY
UPTAKE
ANTI-INFECTIVE AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
COMPLEXES
COPPER ISOTOPES
DAYS LIVING RADIOISOTOPES
DRUGS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
TRANSITION ELEMENT COMPLEXES
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)
550201 - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques