Specific suppression of anti-DNA production in vitro
To investigate the regulation of anti-DNA antibody production, we generated anti-DNA-specific suppressor cells by exposing normal human T cells and a small percentage of adherent cells to high concentrations of DNA. These cells suppressed the production of anti-DNA by both autologous peripheral blood mononuclear cells (PBMC) and allogeneic PBMC derived from systemic lupus erythematosus (SLE) patients. Anti-DNA production was suppressed significantly more than anti-RNA, antitetanus, or total immunoglobulin production. Specific suppression was enhanced by increasing the numbers of DNA-primed CD8+ cells and was obliterated by irradiation of the DNA-primed cells. In contrast to T cells from normal individuals, T cells obtained from two intensively studied SLE patients were unable to generate specific suppressor cells for anti-DNA production in both autologous and allogeneic test systems. Despite this defect, these patients were still capable of generating specific suppressor cells for antibody production directed against an exogenous antigen, tetanus toxoid.
- Research Organization:
- Harbor-UCLA Medical Center, Torrance (USA)
- OSTI ID:
- 6599588
- Journal Information:
- J. Clin. Immunol.; (United States), Vol. 8:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANTIBODIES
BIOSYNTHESIS
DNA
GENETIC RADIATION EFFECTS
IMMUNOSUPPRESSION
IN VITRO
IRRADIATION
LYMPHOCYTES
MACROPHAGES
MAN
MONOCYTES
PATIENTS
RHEUMATIC DISEASES
RNA
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
GENETIC EFFECTS
LEUKOCYTES
MAMMALS
MATERIALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
PRIMATES
RADIATION EFFECTS
SKELETAL DISEASES
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture