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Title: Effects of nitrogen dioxide on biochemical and physiological characteristics of soybean (Glycine max (L. ) Merrill)

Thesis/Dissertation ·
OSTI ID:6596611

One month old soybean cv. Williams plants were exposed to nitrogen dioxide (NO/sub 2/ at 0.1, 0.2, 0.3, and 0.5 ppm) and carbon-filtered air (control), 7 hour per day, for 5 days, under controlled environment. Data were collected on net photosynthetic rate (PN), stomatal resistance (SR), and dark respiratory rate (DR), immediately following fifth day of exposure and 24 hours after termination of exposure using LICOR 6000 Portable Photosynthesis System. Chlorophyll a (Ch a), chlorophyll b (Ch b), and total chlorophyll (tot Ch) were measured before and after exposures, using a spectrophotometer. Leaf nitrogen was determined using kjeldahl digestion followed by colorimetric determination of total N. Growth characteristics-relative growth rate (RGR), net assimilation rate (NAR), leaf area ratio (LAR), and root shoot ratio (RSR)-were computed for treated plants using standard growth equations. Solute leakage (electrical conductivity) was measured 2 and 24 hours after incubation of treated leaf tissue in deionized water.

Research Organization:
Maryland Univ., Princess Anne (USA). Coastal Ecology Research Lab.
OSTI ID:
6596611
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English