Comparison of cyclosporine determinations in whole blood by three different methods. HPLC, /sup 125/I RIA and /sup 3/H RIA

Journal Article · Wed Apr 01 00:00:00 EST 1987 · Am. J. Clin. Pathol.; (United States)

DOI:https://doi.org/10.1093/ajcp/87.4.528· OSTI ID:6593308

Huang, W Y; Lipsey, A I; Cheng, M H

The authors have analyzed and compared the cyclosporine concentrations in whole blood specimens from pediatric renal transplant patients using three different methods: high-performance liquid chromatography (HPLC) (5u C18 reverse-phase column), /sup 3/H radioimmunoassay (RIA), and /sup 125/I RIA (substituted /sup 3/H-tracer in Sandoz Kit with /sup 125/I tracer. Results obtained by the /sup 125/I RIA correlated well with results obtained by the /sup 3/H RIA. Both RIA methods had similar correlation with the HPLC method. The /sup 125/I RIA method showed higher sensitivity and greater precision than the /sup 3/H RIA method. The authors conclude that the /sup 125/I RIA method can be used for cyclosporine determination in whole blood specimens. The use of the /sup 125/I RIA provides a simple and rapid method with higher counting efficiency and less background quenching than the /sup 3/H RIA method, which requires cumbersome liquid scintillation counting procedures.

• Research Organization: Children's Hospital of Los Angeles, CA
• OSTI ID: 6593308
• Journal Information: Am. J. Clin. Pathol.; (United States), Vol. 4
• Country of Publication: United States
• Language: English

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