NMR analysis of sequence of toxin II from the sea anemone Radianthus paumotensis

Journal Article · Tue Nov 04 00:00:00 EST 1986 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00370a017· OSTI ID:6590643

Wemmer, D E; Kumar, N V; Metrione, R M; Lazdunski, M; Drobny, G; Kallenbach, N R

Toxin II from Radianthus paumotensis (Rp/sub II/) has been investigated by high-resolution NMR and chemical sequencing methods. Resonance assignments have been obtained for this protein by the sequential approach. NMR assignments could not be made consistent with the previously reported primary sequence for this protein, and chemical methods have been used to determine a sequence with which the NMR data are consistent. Analysis of the 2D NOE spectra shows that the protein secondary structure is comprised of two sequences of ..beta..-sheet, probably joined into a distorted continuous sheet, connected by turns and extended loops, without any regular ..cap alpha..-helical segments. The residues previously implicated in activity in this class of proteins, D8 and R13, occur in a loop region.

• Research Organization: Univ. of California, Berkeley
• OSTI ID: 6590643
• Journal Information: Biochemistry; (United States), Vol. 25:22
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
TOXINS
NUCLEAR MAGNETIC RESONANCE
STRUCTURAL CHEMICAL ANALYSIS
AMINO ACID SEQUENCE
HEAVY WATER
INVERTEBRATES
PROTONS
ANIMALS
ANTIGENS
BARYONS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HYDROGEN COMPOUNDS
MAGNETIC RESONANCE
MATERIALS
MOLECULAR STRUCTURE
NUCLEONS
OXYGEN COMPOUNDS
RESONANCE
TOXIC MATERIALS
WATER
550601* - Medicine- Unsealed Radionuclides in Diagnostics