Induction of stable p53 oncoprotein and of c-myc overexpression in cultured normal human uroepithelium by radiation and N-nitrosodiethanolamine

Journal Article · Fri Apr 01 00:00:00 EST 1994 · Radiation Research; (United States)

DOI:https://doi.org/10.2307/3578851· OSTI ID:6590526

Mothersill, C; Seymour, C B ^[1]; Harney, J ^[2]

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1. Dublin Institute of Technology, Dublin (Ireland)
2. Beaumont Hospital, Dublin (Ireland)

Uroepithelium cultured from normal patients without cancer (60 individuals) was found to segregate into four subtypes based on the level of carcinogen treatment needed to induce abnormal p53 and c-myc. Twenty-two percent of patient cultures never showed abnormal p53 expression, even after chronic exposure to nitrosamines, while a further 26% required only a single dose of radiation to induce the abnormal protein. The remaining patients had tissues which, while initially negative for stable p53, became positive when put into culture and stimulated to grow. The c-myc protein was overexpressed in all cultures with abnormal p53. It would appear that elevated expression of conformationally inactive p53 and of high levels of c-myc represents an early response of normal uroepithelial cells to carcinogen challenge. It also appears that a relatively high number of patients without cancer express these proteins when their cells are challenged to grow; a pre-exposure to environmental carcinogens such as nitrosamines in cigarette smoke is likely to be involved. 30 refs., 3 figs., 3 tabs.

• OSTI ID: 6590526
• Journal Information: Radiation Research; (United States), Vol. 138:1; ISSN 0033-7587
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BLADDER
ONCOGENIC TRANSFORMATIONS
DNA REPAIR
GENE REGULATION
NITROSAMINES
CARCINOGENESIS
CHRONIC EXPOSURE
RADIOINDUCTION
CARCINOGENS
COBALT 60
AMINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL TRANSFORMATIONS
COBALT ISOTOPES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MINUTES LIVING RADIOISOTOPES
NITROSO COMPOUNDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
RADIOISOTOPES
REPAIR
URINARY TRACT
YEARS LIVING RADIOISOT
560300* - Chemicals Metabolism & Toxicology
560151 - Radiation Effects on Animals- Man
550200 - Biochemistry